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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 品系：
**
- 细胞类型：
**
- 肿瘤类型：
**
- 供应商：
北京智杰方远科技有限公司
- 英文名：
StemPro™ Osteogenesis Differentiation Kit
- 生长状态：
良好
- 年限：
1年
- 运输方式：
-20℃
- 器官来源：
**
- 是否是肿瘤细胞：
否
- 细胞形态：
良好
- 免疫类型：
**
- 物种来源：
**
- 相关疾病：
**
- 组织来源：
**
- 规格：
套
stempro软骨分化试剂盒，骨髓间充质干细胞（MSCs）软骨分化
诱导间充质干细胞分化成软骨细胞试剂盒GIBCO:A1007101
描述
The StemPro® Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the chondrogenesis pathway and generate chondrocytes. Using StemPro® Chondrogenesis Differentiation Kit in combination with StemPro® MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion, and differentiation into collagen matrix-producing chondrocytes.
规格
Cell Type: Stem Cells (Mesenchymal)
分类: Serum-Free
Culture Environment: CO2
Endotoxin Level: ≤50 EU/mL, ≤ 50 EU/ml
外形: Liquid
Glucose: Low Glucose
Glutamine: GlutaMAX™-I
HEPES Buffer: No HEPES
Phenol Red Indicator: No Phenol Red
Product Line:
Purity or Quality Grade: Research Grade
Research Category: Differentiation
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
种类: Human
内容及储存
This kit is shipped with a 100 ml basal medium and 10 ml growth supplement adequate to formulate 100 ml of complete medium. Components require separate shipping and storage requirements. The growth supplement is not sold individually.
StemPro® Osteocyte⁄Chondrocyte Differentiation Basal Medium 2 to 8°C (protect from light)
StemPro® Chondrogenesis Supplement -5 to -20°C (in the dark)
诱导间充质干细胞分化成软骨细胞试剂盒GIBCO:A1007101
| 品名 | 品牌 | 货号 | 规格 | 单位 | 价格 |
| StemPro™ Osteogenesis Differentiation Kit | GIBCO | A1007101 | 1 kit | 套 | 询价 |
The StemPro® Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the chondrogenesis pathway and generate chondrocytes. Using StemPro® Chondrogenesis Differentiation Kit in combination with StemPro® MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion, and differentiation into collagen matrix-producing chondrocytes.
规格
Cell Type: Stem Cells (Mesenchymal)
分类: Serum-Free
Culture Environment: CO2
Endotoxin Level: ≤50 EU/mL, ≤ 50 EU/ml
外形: Liquid
Glucose: Low Glucose
Glutamine: GlutaMAX™-I
HEPES Buffer: No HEPES
Phenol Red Indicator: No Phenol Red
Product Line:
Purity or Quality Grade: Research Grade
Research Category: Differentiation
Serum Level: Serum-Free
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
种类: Human
内容及储存
This kit is shipped with a 100 ml basal medium and 10 ml growth supplement adequate to formulate 100 ml of complete medium. Components require separate shipping and storage requirements. The growth supplement is not sold individually.
StemPro® Osteocyte⁄Chondrocyte Differentiation Basal Medium 2 to 8°C (protect from light)
StemPro® Chondrogenesis Supplement -5 to -20°C (in the dark)
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文献和实验相关实验
R&D Systems:如何提高MSC的扩增且不损失其分化潜能
。为了评估不同来源和浓度的血清对MSC扩增的影响，我们用市上分化试剂盒和组分严格地测试了每种FBS对最大扩增潜能，维持hMSC表型特性和扩增的MSCs分化为脂肪细胞，骨细胞，软骨细胞的影响。我们通过对FBS的筛选开发了StemXVivo人/小鼠骨髓间充质干细胞扩增培养基。 我们的研究强调利用为研究hMSCs而开发，优化和测试的市上产品的优点。 我们的研究强调使用商品化产品的优势。为了hMSCs的研究，这些商品化的产品都已经被改善，优化，并且进行了检测。 材料和方法 人MSC 的培养
相关的检测,同时按同样的密度进行传代。 兔骨髓间充质干细胞的传达培养:将传代的第P1 代、第P4 代、第P8 代间充质干细胞,按每孔2 ×105个定量接种于24 孔培养板,次日起,每天取3 孔的细胞,经0.25 %胰蛋白酶消化后,作细胞计数,直至基本铺满整个瓶底。按3 孔细胞均数取点,绘制其生长曲线。传代培养过程中,隔日换液。 实验研究用Percoll (密度为1.073g/ ml) 梯度离心法加贴壁培养方法提取MSCs 既是简便的,又是实用的,与Ficoll (密度为1.077g/ ml) 梯度
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GIBCO成软骨分化试剂盒A1007101，骨髓间充质干细胞MSCs成软骨分化
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