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- PTVB00172-2a
- 2025年09月20日
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- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pcDNA3.1(+)-FASN
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pcDNA3.1(+)-FASN/ pcDNA3.1(+)-FASN 璐ㄧ矑
pcDNA3.1(+)-FASN 2ug
Catalog number PTVB00172-2a
Plasmid name: pcDNA3.1(+)-FASN
Quantity: Please refer to the label (tube)
Gene/insert name: FASN
Alt name: FAS; OA-519; SDR27X1
Insert size: 7536bp
Species: Homo sapiens (human)
Gene ID: 2194
Accessions: NM_004104
Vector Backbone: pcDNA3.1(+)
Tag: None
Backbone manufacturer: Invitrogen
Vector type: Mammalian Expression
Backbone size w/o insert: 5428bp
Cloning site 5': Kpn I
Site destroyed during cloning: NO
Cloning site 3': EcoR I
Site destroyed during cloning: NO
Bacterial resistance(s): Ampicillin
Growth strain(s): Top10
Growth temperature: 37
High or low copy: High Copy
Plasmid description: Mammalian Expression vector of Homo sapiens fatty acid synthase
Selectable markers: Neomycin
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文献和实验wangch84 各位高手，兄弟最近忙于质粒构建，意图将一段长约4000 bp的片段，通过BamH1和EcoR1两个酶切位点连接到pcDNA3.1+载体上。情况如下： 1. 通过LA Taq酶扩增，并纯化回收目的片段（引物中带有酶切位点，保护性碱基＝3，上游带有Kozak序列）；由于回收效率很高，所以在进行下一步双酶切时只加了12 μl的目的片段，H2O 20 μl，10 X K BUFFER 4 μl，两种酶各2 μl，37℃作用过夜（12-14 h
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
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