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- 文献和实验
- 技术资料
- 保存条件：
4°C, sealed storage, away from moisture and light
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
201998-61-0
- 规格：
5 mg/10 mg/25 mg/50 mg
| 规格： | 5 mg | 产品价格： | ￥720.0 |
|---|---|---|---|
| 规格： | 10 mg | 产品价格： | ￥1236.0 |
| 规格： | 25 mg | 产品价格： | ￥2400.0 |
| 规格： | 50 mg | 产品价格： | ￥4104.0 |
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Py-BODIPY-NHS ester
CAS No. : 201998-61-0
MCE 国际站：Py-BODIPY-NHS ester
产品活性：BODIPY 染料是一种具有强紫外吸收能力的小分子染料，其荧光峰比较尖锐，量子产率高。其对环境的极性和 pH 值相对不敏感，因此，在不同生理条件下相对稳定。由于其结构的非对称性， BODIPY 具有多种衍生结构产物。BODIPY 脂滴类染料可以很好的穿过细胞膜进入细胞内部，通过在细胞内的极性脂类上定位以进行特异性染色，因此，该染料可用于标记活细胞和固定细胞。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: General Protocol (Example for Py-BODIPY NHS ester)
1.Preparation of Py-BODIPY NHS ester working solution
1.1Preparation of the stock solution
Dissolve 1 mg Py-BODIPY NHS ester in 382 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20 ℃ -80 ℃ away from light and avoid repetitive freeze-thaw cycles.
1.2Preparation of BODIPY 493/503 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Py-BODIPY NHS ester working solution according to the actual situation.
2.Cell staining
2.1 Suspension cells (6-well plate)
a.Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106mL.
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c.Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant.
d.Wash twice with PBS, 5 minutes each time.
e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry.
相关产品：MitoSOX Red | BODIPY 493/503 | Nile Red | D-Luciferin potassium | JC-1 | FITC | Propidium Iodide | CFDA-SE | FITC-Dextran (MW 4000) | Dihydroethidium | DiR | Calcein-AM | Thiazolyl Blue | 2-NBDG | DiI | Fluo-4 AM | TMRE | Cy5.5 | Indocyanine green | Acridine Orange hydrochloride | CY5 | BODIPY 581/591 C11 | FM4-64 | CY5-SE | PKH 26 | DiD perchlorate | Diphenylterazine
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文献和实验International (Cupertino, CA). BSA-NHS slides, displaying activated amino and carboxyl groups on the surface of an immobilized layer of bovine serum albumin (BSA), were fabricated as follows. 10.24 g N,N'-disuccinimidyl carbonate (100 mM) and 6.96 ml N,N
芯空一号快讯 | 跟SBC一起做科研：趣味厘清ICB耐药机制研究
2⁺ LAM到底是不是真的在给肿瘤供脂，光靠相关性不够吧？上动物模型了吗？ 蒋老师： 上了，而且设计得特别干净。他们先用抗PD-1反复筛选建出了ICB耐药的小鼠HCC模型，然后拿多重免疫荧光直接看空间分布，发现脂滴全堆在肿瘤实质区域，TREM2⁺ LAM密集的地方脂质密度高出5倍以上。这些巨噬细胞本身BODIPY染色也更强，转录组里脂肪酸转运和胞葬通路全在跑。然后他们做了一个很漂亮的因果实验：只在巨噬细胞里敲掉Trem2，别的细胞一概不动，再种耐药肿瘤。用一种叫SCENITH的方法直接测癌
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
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