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- 2025年12月05日
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- 保存条件：
-20°C, sealed storage, away from moisture and light
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- CAS号：
325143-98-4
- 规格：
5 mg
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Sulfo-NHS-SS-Biotin sodium
CAS No. : 325143-98-4
MCE 国际站：Sulfo-NHS-SS-Biotin sodium
产品活性：Sulfo-NHS-SS-biotin 是一种长链可裂解和细胞无渗透的胺类活性生物素化 (biotinylation) 试剂。Sulfo-NHS-SS-biotin 可用于细胞表面蛋白 (cell-surface protein) 的标记和纯化。
研究领域：Others
作用靶点：Fluorescent Dye
In Vitro: Sulfo-NHS-SS-biotin is a cell-surface-labeling reagent,? it is a negatively charged reagent and does not permeate cell membranes.
?Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides.
?Sulfo-NHS-SS-biotin has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.
?Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by? ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer.
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and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surface. In the biotinylation method, cell surface receptors are labeled with Sulfo-NHS-biotin. The charge on the sulfonyl
Detection of Tight Junction Barrier Function In Vivo by Biotin
molecules with different sizes have been widely used to determine the TJ barrier function. In the in vivo system, the tracer molecule Sulfo-NHS-Biotin was initially used in Xenopus embryo system and subsequently was successfully applied to a number of animal
下面的操作方法可以使约3~5分子的生物素与1分子的蛋白结合，对某些蛋白来说，生物素与蛋白的分子比可根据不同的生物素化要求进行调整。 1. 溶解2~10 mg 蛋白于1 ml 的BuphTm磷酸盐缓冲液中，并计算溶解的毫摩尔数。如果蛋白含有不恰当的缓冲液（如Tris或者甘氨酸），可以通过在PBS中透析或浓缩的方法除去。计算：蛋白质量（mg）/蛋白分子量（MW）＝蛋白质的毫摩尔数。 2. 在打开之前，平衡生物素至室温。加2 mg Sulfo-NHS-Biotin于100 ul
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