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文献和实验Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis
A screening method is described for determining whether a drug or small solute has significant interactions at the two major binding sites on human serum albumin (HSA). This method uses affinity capillary electrophoresis (ACE) to perform
Serum is a readily available source for diagnostic assays, but the identification of disease-specific serum biomarkers has been impeded by the dominance of human serum albumin (HSA) and immunoglobulin G (IgG) in the serum proteome
又称白蛋白。是一类不被 50%饱和度的硫酸铵溶液沉淀的球状蛋白质。存在于动物组织、体液和某些植物的种子中。其分子量较低,溶于水,易结晶。在中性溶液中加热即沉淀或凝固。其重要代表是血清蛋白、乳清蛋白、卵清蛋白、麦清蛋白、豆清蛋白及有毒的蓖麻蛋白。人血清(或血浆)清蛋白占血清蛋白质的 55~ 63%,是血清中少数不含糖的蛋白质之一;分子量 67500道尔顿,有 584个氨基酸残基和高净电荷(等电点 4.9);与水、 Ca2 、 Na 、 K 、脂肪酸及胆红素都有较好
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