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文献和实验微量凯氏(Micro-Kjeldahl)定氮法测定蛋白质含量
【实验原理】 蛋白质(或其它含氮有机化合物)与浓H2SO4共热时,其中碳、氢两种元素被氧化成CO2和H2O,而氮元素转变成NH3,并进一步与H2SO4反应生成(NH4)2SO4,残留于消化液中,该过程通常称为“消化”。以甘氨酸为例: CH2NH2COOH + 3H2SO4 → 2CO2 + 3SO2 + 4H2O + NH3 2NH3 + H2SO4 → (NH4)2SO4
Quantitation of Protein:Micro-Kjeldahl Method
【 Purpose 】 Study the principle and the operational techniques of micro-Kjeldahl method. 【 Principle 】 The Kjeldahl method measures the nitrogen content of a compound and may be used to determine
Isolation of micro-RNA (miRNA)
the frozen tissue sample in solution D using a Dounce, micro-Dounce, or a mechanical microhomogenizer. 5. Transfer the homogenate to a microcentrifuge tube. Note Hereafter, the volume of homogenate used is defined as "volume D" (e.g., in the sample
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