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文献和实验SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
by inverting the tube a few times. Incubate 5 minutes at room temperature. Invert the tube several times during incubation. NOTE: ERL Buffer is supplied as a 10x concentrate and must be diluted with ddH2 O according to bottle label. 2. Centrifuge
RNA Purification Protocol for 10-20mg of Animal Tissue
. Fresh tissue can also be used. Work quickly and keep the sample on ice at all time. 2. Add 10-20 mg frozen ground tissue or fresh tissue to a 1.5 mL microtube containing 600ul RCL Buffer. Homogenize throughly using a microfuge tube pestle (Product
IASYS-binding cuvette and getting KD
of ligands to the carboxymethylated dextran coated cuvette (1) Dissolve 0.2 g N-Hydroxysuccinimide (NHS)in 15 ml of deionized H2O to get 0.0133 g/ml (0.116 mol/L)concentration and aliquot into 250 µl volume and store in a -20 ℃ freezer. (2) Dissolve 1.15 g
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