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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 现货状态:
现货
- 规格:
0.22μm
滤膜材质:PES(聚醚砜)
类别:过滤器
产地:进口
材质:塑料
容量:250ml
包装规格:12个/盒
赛多利斯180C7-E 一次性真空过滤装置 SartolabRF250;PES;0,22µm;sterile;12St
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文献和实验at 60oC ). Flicking the bottom of tubes ensured that all the DNA has been suitably resuspended. 2) The samples were then given a quick spin in a microcentrifuge and kept on ice prior to use. 3) 1µl of the DNA sample + 2µl loading dye + 10 µl sterile
volume with distilled water 100 mM EDTA : 20 ml 0.5 M EDTA 80 ml ddH2 O 100 ml 95% ethanol/0.12 M NaOAc (ethanol/acetate): 95 ml 100% ethanol 4 ml 3 M NaOAc pH 4.5 1 ml ddH2 O 100 ml 5 mg/ml ethidium bromide (EtBr): 500 mg EtBr (Sigma E-8751) ddH2 O
filter sterililize; no DEPC treatment. Preparation of dNTP's. The quick protocol for ~100mM stock. dilute all four dNTP's (250mg of each) in 3.676ml of H2 O; + 424µl 5M NaOH; check the pH: ~0.5µl on pH-paper
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