- ￥1560
- Sino Biological
- 北京
- 50945-M07HL
- 2025年08月12日
- WB
- HEK293 Cells
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A DNA sequence encoding the mouse CLEC4A (NP_001163803.1) (Gln70-Leu238) was expressed with a polyhistidine tag at the N-terminus.
- 亚型：
见说明书
- 形态：
液体
- 保存条件：
4℃
- 克隆性：
无
- 标记物：
见说明书
- 适应物种：
Mouse
- 保质期：
12个月
- 抗原来源：
见说明书
- 目录编号：
50945-M07HL
- 级别：
免疫学
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 宿主：
HEK293 Cells
- 应用范围：
WB
- 浓度：
见说明书
- 靶点：
CLEC4A
- 抗体英文名：
mouse DCIR / CLEC4A / CLECSF6 CHO Cell Lysate (WB positive control)
- 抗体名：
mouse DCIR / CLEC4A / CLECSF6 CHO Cell Lysate (WB positive control)
- 规格：
300 µg
反应种属：Mouse
裂解液靶点：CLEC4A
裂解液应用：WB
裂解液保存条件：Store at 4℃. After re-dissolution, aliquot and store at -80℃.
裂解液产品描述：HEK293 Cell mouse DCIR / CLEC4A / CLECSF6 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
裂解液表达宿主：HEK293 Cells
裂解液制备方法：Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
裂解液Buffer：Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
裂解液质控信息：12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
裂解液使用建议：1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
裂解液稳定性：Samples are stable for up to twelve months from date of receipt.
裂解液使用说明：Western blot (WB): Use at an assay dependent dilution. Other Applications: Not tested.
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文献和实验Using the DFCI Gene Index Databases for Biological Discovery
functional classes through Gene Ontology assignments. This protocol provides guidance for using the Gene Index Databases to extract information. Curr. Protoc. Bioinform. 29:1.6.1?1.6.36. © 2010 by John Wiley & Sons, Inc. Keywords: gene
. Functional validationof targeting constructs bearing the designed guides canbe carried out in relevant cell lines, e.g., HEK 293FT, K562,Hela for human genome engineering, or Neuro-2a, Hepa1-6for mouse. This process takes advantage
：表达产物 蛋白名称（可保密）： 蛋白分子量：29 KDa WB用膜类型、孔径：PVDF 转膜方式（恒压、恒流）：0.3 A 恒流 转膜时间：80 min 转膜设备: 湿转 Bio-Rad 蛋白来源：cell lysate, culture medium 蛋白名称（可保密）： 蛋白分子量：15-50 kD WB用膜类型、孔径：NC，PVDF， 0.45 um 转膜方式（恒压、恒流）：恒压， semi
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