PBST (10×,pH7.4)
PBST(phosphate buffered solution)即含吐温-20的磷酸盐缓冲液,能够提供相对稳定的离子环境和pH缓冲能力,是生物学中经常使用的缓冲盐溶液,主要用于免疫组化和原位杂交,酶联免疫等实验中,清洗免疫印迹膜等。产品为无色液体,pH为7.2-7.4(25℃),主要成分为磷酸二氢钾、磷酸氢二钠、氯化钠以以及吐温-20。
本产品为10×原液,不可直接使用,需要稀释到1×工作液使用。 4℃保存可延长产品保质期,长期不用建议低温保存。
注意事项
低温储存较易产生沉淀。如果发现有沉淀产生,请摇晃或置于37℃水浴至完全溶解后再使用。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
A 10X Concentrated solution of Phosphate Buffered Saline with Tween® 20. The 10X Concentration is 80mM Na2HPO4, 1.5M NaCl, 20mM KH2PO4, 30mM KCl, 0.5% Tween® 20, pH 7.4.
PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The Phosphate Buffered Saline with Tween® 20 is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.
By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Features
1X Concentration is 8mM Na2HPO4, 150mM NaCl, 2mM KH2PO4, 3mM KCl, 0.05% Tween® 20, pH 7.4
Applications
Wash buffer for Western Blotting and ELISA