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文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Screening cDNA Libraries Using Partial Probes to Isolate Full-Length cDNAs from Vascular Cells
within the library selected (1 ,2 ). As an example of both the utility and power of library screening, we will relate our own library screening efforts utilized to isolate the nonmuscle high molecular weight myosin light chain kinase isoform from a human umbilical
into the fertilized egg at the earliest stage, which is the pronuclear period immediately following fertilization. For several hours following the entry of the sperm into the oocyte, the male and the female pronuclei are visible as individuals under normal light
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