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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
has been demonstrated for Drosophila Wingless (12) ,Xenopus Wnt8 (17) , and mammalian Wnt1 (10) . We haveused mammalian Wnt3a in our studies. 4. Many commercial antibodies that recognise phosphorylatedand non-phosphorylated epitopes on GSK3 are listed in Table
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
, and differentiation. More than 50 GSK-3 inhibitors representing a wide range of chemical structures have already been identified,and their utility in the treatment of type II diabetes mellitus, Alzheimer’s disease, bipolar diseases, cancer, andother human
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