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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
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Please store the product under the recommended conditions in the Certificate of Analysis.
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MedChemExpress LLC
- CAS号:
1392488-04-8
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询盘
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NIR-H2O2
CAS No. : 1392488-04-8
MCE 国际站:NIR-H2O2
产品活性:NIR-H2O2 是一种可透过细胞的 NIR 荧光启动传感器。NIR-H2O2 在 NIR 区域内同时具有吸收和发射作用。当在 NIR 区域激发时,NIR-H2O2 对 H2O2 产生较大的启动 NIR 荧光信号。NIR-H2O2 能够对活细胞和小鼠中内源性产生的 H2O2 进行成像。
研究领域:Others
作用靶点:Fluorescent Dye
In Vitro: NIR-H2O2 is highly selective to H2O2 over other typical ROS and biorelevant species.
HeLa cells incubated with NIR-H2O2 (5 μM) for 30 min at 37 °C provide almost no fluorescence. However, when the living HeLa cells loaded with NIR-H2O2 are further treated with H2O2, they give strong fluorescence. NIR-H2O2 is cell membrane permeable and responsive to H2O2 in the living cells. When stimulated by phorbol myristate acetate (PMA), macrophage cells may produce endogenous H2O2. The living RAW264.7 macrophage cells loaded with only the NIR sensor NIR-H2O2 (1 μM) display almost no fluorescence. However, the macrophage cells coincubated with PMA (3.0 μg/mL) and the sensor NIR-H2O2 (1 μM) exhibit a dramatic enhancement in the red emission. NIR-H2O2 is capable of fluorescent imaging of endogenously produced H2O2 in the living RAW264.7 macrophage cells. The mitochondria staining experiments suggest that the sensor mainly associates with the mitochondria of RAW264.7 macrophage cells.
In Vivo: The H2O2 production in vivo was generated by activated macrophages and neutrophils in a lipopolysaccharide (LPS) model of acute inflammation. The mice treated with both LPS and NIR-H2O2 exhibit a significantly higher fluorescence readout than the mice untreated or treated with only NIR-H2O2. The mice loaded with LPS and NIR-H2O2 have approximately 10- and 20-fold higher fluorescence intensity than the mice loaded with saline and the sensor and the mice loaded with saline, respectively.
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化物,室温10~20分钟。4.正常血清(1:10)孵育,室温20分钟。5.滴加第一抗体,37℃1小时或4℃过夜。6.PBS洗涤3×3分钟。7.滴加酶标二抗,室温1小时。8.PBS洗涤3×3分钟。9.0.04%DAB+0.03 %H2O2显色5~10分钟。10. 充分水洗后,复染、脱水、透明、封片、镜检。三、PAP法(过氧化物酶抗过氧化物酶抗体复合物法)1.标定固定后,PBS洗涤。2.1% H2O2(或1% H2O2甲醇)阻断内源性过氧化物,室温10~20分钟。3.PBS洗涤2×3分钟。4.正常血清(二抗
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