- 询价
- Sigma-Aldrich
- ZRB1147-4X25UL
- 2025年10月18日
- Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry
- rabbit
- Human, Mouse. Predicted to react with Rat based on 100% sequence homology.
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 克隆性：
单克隆
- 适应物种：
Human, Mouse. Predicted to react with Rat based on 100% sequence homology.
- 库存：
大量
- 供应商：
默克生命科学
- 宿主：
rabbit
- 应用范围：
Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry
- 靶点：
Calnexin
- 抗体英文名：
ANTI-CALNEXIN,CLONE10N19 ZOOMAB RB MONO
- 规格：
4X25UL
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文献和实验Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
Protocol for anti-HA antibody Western Blotting
it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
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