- 询价
- Sigma-Aldrich
- ZRB5320-25UL
- 2025年10月16日
- Immunocytochemistry;Western Blotting;Immunofluorescence
- rabbit
- Mouse, Rat.
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 克隆性：
单克隆
- 适应物种：
Mouse, Rat.
- 库存：
大量
- 供应商：
默克生命科学
- 宿主：
rabbit
- 应用范围：
Immunocytochemistry;Western Blotting;Immunofluorescence
- 靶点：
Chondroitin sulfate proteoglycan 4
- 抗体英文名：
ANTI-NG2, CLONE 1L2, ZOOMAB RABBIT MONO
- 规格：
25UL
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
m g/mL DNAse I, 30mM Trizma base (pH 7.2), 140mM K cacodylate, 4mM MgCl2 , 0.1mM dithiothreitol 0°C Appendix 2: Reagents 3-amino-9-ethylcarbazole (AEC) Sigma Aldrich
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
Protocol for intracytoplasmic staining of cytokines for FACS analysis
-antiglobulin based detection as below it is advisable to use 'Fc block' (anti-FCgRII/III: 2.4G2; Pharmingen 01241A). 5) Wash cells and fix with 2% v/v formaldehyde in PBS (20min, 4 deg C). 6) Wash cells and permeabilized with PBS + 0.5% saponin (Sigma
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