Anti-C-EBP alpha, cl 2E17 ZooM

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure

免疫球蛋白的类型

） 五类(Class)：（ H链C区的不同氨基酸组成和排列顺序） IgG --- γ(gamma) / 330aa IgA --- α(alpha) / 330aa IgM --- μ(mu) / 440aa IgD --- δ(delta) / 330aa IgE  ---ε(epsilon) / 440aa 亚类(Subclasses)：（ 同一类IgH链C区，氨基酸组成的较小差异/二硫键位置或数目不同） IgG：IgG1,  IgG

Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells

can also be substituted. TWEEN is often used in whole embryos. Make solutions with detergent fresh on the day of use. TBS (For all steps after fixing. ) 0.15 M NaCl 0.02 M Tris-Cl pH 7.4 Can make 10X stocks. Keep at room temperature or 4¡C. PBS