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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
- 亚型:
IgG, Fc fragment
- 形态:
Freeze-dried solid
- 保存条件:
Store freeze-dried solid at 2-8°C.
- 克隆性:
单克隆
- 标记物:
DyLight™ 405
- 适应物种:
Goat
- 保质期:
One year from date of rehydration.
- 抗原来源:
IgG, Fc fragment
- 供应商:
Jackson ImmunoResearch Laboratories Inc.
- 宿主:
Rabbit
- 抗体英文名:
DyLight 405-AffiniPure Rabbit Anti-Goat IgG, Fc Fragment Specific (min X Hu Sr Prot)
- 抗体名:
AffiniPure Whole IgG
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文献和实验这类抗体只与 IgG 重链反应,通常不能和 IgA 或 IgM 任何表位反应。 注意:不是所有 anti-IgG, Fc 抗体和所有亚型的 IgG 反应能力一致。要找和四种 IgG 亚类反应能力差不多的 抗鼠IgG, Fc γ抗体的话,应当选羊抗鼠IgG( 亚类1+2a+2b+3), Fc γ特异性片段(min X Hu,Bov,Rb Sr Prot) 。 Anti-IgG , F(ab ’)2 fragment specific 这类抗体和 IgG 的 F(ab
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
Incubate the plate overnight at 4°C (or for 2-3 hr at 30°C). Wash the plate extensively (3 x 5 min) with 300 µl/well of HBST. Dilute goat anti-rabbit IgG coupled to AP 5,000-10,000
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