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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
- 亚型:
IgG, Fc fragment
- 形态:
Sterile-filtered liquid
- 保存条件:
Store at 2-8°C under sterile conditions.
- 克隆性:
单克隆
- 标记物:
Unconjugated
- 适应物种:
Goat
- 保质期:
One year from date of receipt.
- 抗原来源:
IgG, Fc fragment
- 供应商:
Jackson ImmunoResearch Laboratories Inc.
- 宿主:
Rabbit
- 抗体英文名:
AffiniPure F(ab')2 Fragment Rabbit Anti-Goat IgG, Fc Fragment Specific (min X Hu Sr Prot)
- 抗体名:
AffiniPure F(ab')2 Fragment
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文献和实验Site-Specific Chemical Modification of a Glycoprotein Fragment Expressed in Yeast
immunoglobulin G subclass 1 fragment crystallizable (IgG1 Fc), by native chemical ligation. The glycosylated IgG1 Fc is expressed in a glycosylation-deficient yeast strain. The N-terminal cysteine is generated by the endogenous yeast protease Kex2 in the yeast
T-cell dependent antigen specific in situ staining (Pe)
-conjugated secondary Ab choice: 10- add the biotin-conjugated goat anti-rabbit (50 to 100 µl, dilution 1:200) and incubate for 30 min. 11- wash thrice in TBS-T. From this step on follow either "Development (Avidin-HRP) " or "Double staining
这类抗体只与 IgG 重链反应,通常不能和 IgA 或 IgM 任何表位反应。 注意:不是所有 anti-IgG, Fc 抗体和所有亚型的 IgG 反应能力一致。要找和四种 IgG 亚类反应能力差不多的 抗鼠IgG, Fc γ抗体的话,应当选羊抗鼠IgG( 亚类1+2a+2b+3), Fc γ特异性片段(min X Hu,Bov,Rb Sr Prot) 。 Anti-IgG , F(ab ’)2 fragment specific 这类抗体和 IgG 的 F(ab
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