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- 规格:
10ml
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| ST506 | PMSF (100mM) | 10ml | 112.00元 |
PMSF即Phenylmethanesulfonyl fluoride。分子式为C7H7FO2S,分子量为174.19,纯度
>99%。
常用生化试剂,用于抑制蛋白酶,进口试剂配制。
第一次使用时把一管PMSF晶体全部倒入到PMSF溶剂中,溶解混匀,即配制成10毫升100mM PMSF。
包装清单:
| 产品编号 | 产品名称 | 包装 |
| ST506-1 | PMSF (晶体) | 可以配制10毫升 |
| ST506-2 | PMSF (溶剂) | 10ml |
| — | 说明书 | 1份 |
保存条件:
室温保存,配成PMSF溶液后需-20℃保存。
注意事项:
本产品对人体有毒,操作时请特别小心,并注意有效防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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文献和实验1. Ye Qi, Li Ding, Siwen Zhang, Shengze Yao, Jennie Ong, Yi Li, Hong Wu, Peng Du . A plant immune protein enables broad antitumor response by rescuing microRNA deficiency Cell. 2022 May 26;185(11):1888-1904.e24. doi: 10.1016/j.cell.2022.04.030. (IF 38.637)
2. Jia Xiong, Jia He, Jun Zhu, Jiongli Pan, Weijie Liao, Hongying Ye, Haofei Wang, Yinjing Song, Yue Du, Bijun Cui, Maoguang Xue, Wanling Zheng, Xiangxing Kong, Kai Jiang, Kefeng Ding, Lihua Lai, Qingqing Wang . Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells Mol Cell. 2022 May 5;82(9):1660-1677.e10. doi: 10.1016/j.molcel.2022.02.033. (IF 15.584)
3. Feixiang Bao, Lingyan Zhou, Rui Zhou, Qiaoying Huang, Junguo Chen, Sheng Zeng, Yi Wu, Liang Yang, Shufang Qian, Mengfei Wang, Xueying He, Shan Liang, Juntao Qi, Ge Xiang, Qi Long, Jingyi Guo, Zhongfu Ying, Yanshuang Zhou, Qiuge Zhao, Jiwei Zhang, Di Zhang, Wei Sun, Mi Gao, Hao Wu, Yifan Zhao, Jinfu Nie, Min Li, Quan Chen, Jiekai Chen, Xiao Zhang, Guangjin Pan, Hong Zhang, Mingtao Li, Mei Tian, Xingguo Liu . Mitolysosome exocytosis, a mitophagy-independent mitochondrial quality control in flunarizine-induced parkinsonism-like symptoms Sci Adv. 2022 Apr 15;8(15):eabk2376. doi: 10.1126/sciadv.abk2376. (IF 13.116)
4. Fu-Hui Xiao, Qin Yu, Zhi-Li Deng, Ke Yang, Yunshuang Ye, Ming-Xia Ge, Dongjing Yan, Hao-Tian Wang, Xiao-Qiong Chen, Li-Qin Yang, Bin-Yu Yang, Rong Lin, Wen Zhang, Xing-Li Yang, Lei Dong, Yonghan He, Jumin Zhou, Wang-Wei Cai, Ji Li, Qing-Peng Kong . ETS1 acts as a regulator of human healthy aging via decreasing ribosomal activity Sci Adv. 2022 Apr 29;8(17):eabf2017. doi: 10.1126/sciadv.abf2017. (IF 13.116)
5. Jiao Wu, Zhuan Feng, Liang Chen, Yong Li, Huijie Bian, Jiejie Geng, Zhao-Hui Zheng, Xianghui Fu, Zhuo Pei, Yifei Qin, Liu Yang, Yilin Zhao, Ke Wang, Ruo Chen, Qian He, Gang Nan, Xuejun Jiang, Zhi-Nan Chen, Ping Zhu . TNF antagonist sensitizes synovial fibroblasts to ferroptotic cell death in collagen-induced arthritis mouse models Nat Commun. 2022 Feb 3;13(1):676. doi: 10.1038/s41467-021-27948-4. (IF 12.121)
6. Mengci Li, Shouli Wang, Yitao Li, Mingliang Zhao, Junliang Kuang, Dandan Liang, Jieyi Wang, Meilin Wei, Cynthia Rajani, Xinran Ma, Yajun Tang, Zhenxing Ren, Tianlu Chen, Aihua Zhao, Cheng Hu, Chengxing Shen, Weiping Jia, Ping Liu, Xiaojiao Zheng, Wei Jia . Gut microbiota-bile acid crosstalk contributes to the rebound weight gain after calorie restriction in mice Nat Commun. 2022 Apr 19;13(1):2060. doi: 10.1038/s41467-022-29589-7. (IF 12.121)
7. Xiaojie Ma, Jie Cao, Ziyu Zhou, Yunkun Lu, Qin Li, Yan Jin, Guo Chen, Weiyun Wang, Wenyan Ge, Xi Chen, Zhensheng Hu, Xiao Shu, Qian Deng, Jiaqi Pu, Chengzhen Liang, Junfen Fu, Jianzhao Liu, Saiyong Zhu . N6-methyladenosine modification-mediated mRNA metabolism is essential for human pancreatic lineage specification and islet organogenesis Nat Commun. 2022 Jul 18;13(1):4148. doi: 10.1038/s41467-022-31698-2. (IF 12.121)
8. Liang Yong, Yafen Yu, Bao Li, Huiyao Ge, Qi Zhen, Yiwen Mao, Yanxia Yu, Lu Cao, Ruixue Zhang, Zhuo Li, Yirui Wang, Wencheng Fan, Chang Zhang, Daiyue Wang, Sihan Luo, Yuanming Bai, Shirui Chen, Weiwei Chen, Miao Liu, Jijia Shen, Liangdan Sun . Calcium/calmodulin-dependent protein kinase IV promotes imiquimod-induced psoriatic inflammation via macrophages and keratinocytes in mice Nat Commun. 2022 Jul 22;13(1):4255. doi: 10.1038/s41467-022-31935-8. (IF 12.121)
9. Wei Li, Qi Long, Hao Wu, Yanshuang Zhou, Lifan Duan, Hao Yuan, Yingzhe Ding, Yile Huang, Yi Wu, Jinyu Huang, Delong Liu, Baodan Chen, Jian Zhang, Juntao Qi, Shiwei Du, Linpeng Li, Yang Liu, Zifeng Ruan, Zihuang Liu, Zichao Liu, Yifan Zhao, Jianghuan Lu, Junwei Wang, Wai-Yee Chan, Xingguo Liu . Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation Nat Commun. 2022 Dec 2;13(1):7414. doi: 10.1038/s41467-022-35199-0. (IF 12.121)
10. Feng S, Cong S, Zhang X, Bao X, Wang W, Li H, Wang Z, Wang G, Xu J, Du B, Qu D, Xiong W, Yin M, Ren X, Wang F, He J, Zhang B. . MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis inlung cancer cells. Nucleic Acids Res. 2011 Aug;39(15):6669-78. (IF 11.501)
11. Xu Y, Dong S, Zhou Q, Mo X, Song L, Hou T, Wu J, Li S, Li Y, Li P, Gan Y, Xu J. . The effect of mechanical stimulation on the maturation of TDSCs-poly(L-lactide-co-e-caprolactone)/collagen scaffold constructs for tendon tissue engineering. Biomaterials. 2014 Mar;35(9):2760-72. (IF 10.317)
12. Yu X, Tang J, Wang Q, Ye W, Tao K, Duan S, Lu C, Yang X, Dong S, Zheng X, Wang Y. . The RxLR effector Avh241 from Phytophthora sojae requires plasma membrane localization toinduce plant cell death. New Phytol. 2012 Oct;196(1):247-60. (IF 8.512)
13. Xiaoxue Lu, Qiaolin Xu, Yanan Tong, Zhujun Zhang, Guodong Dun, Yuyang Feng, Jie Tang, Dan Han, Yilan Mao, Ling Deng, Xiaoyi He, Qian Li, Yang Xiang, FengChao Wang, Dongzhu Zeng, Bin Tang, Xuhu Mao . Long non-coding RNA EVADR induced by Fusobacterium nucleatum infection promotes colorectal cancer metastasis Cell Rep. 2022 Jul 19;40(3):111127. doi: 10.1016/j.celrep.2022.111127. (IF 8.109)
14. Yingchun Wang, Yuzhen Wang, Lu Ding, Xiaolei Ren, Bo Wang, Liyuan Wang, Songbo Zhao, Xuetian Yue, Zhuanchang Wu, Chunyang Li, Xiaohong Liang, Chunhong Ma, Lifen Gao . Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages Cell Rep. 2022 Nov 29;41(9):111738. doi: 10.1016/j.celrep.2022.111738. (IF 8.109)
15. Yang L, Ping YF, Yu X, Qian F, Guo ZJ, Qian C, Cui YH, Bian XW. . Gastric cancer stem-like cells possess higher capability of invasion and metastasis in associationwith a mesenchymal transition phenotype. Cancer Lett. 2011 Nov 1;310(1):46-52. (IF 7.36)
16. Jiao J, Hong S, Zhang J, Ma L, Sun Y, Zhang D, Shen B, Zhu C. . Opsin3 sensitizes hepatocellular carcinoma cells to 5-fluorouracil treatment by regulating theapoptotic pathway. Cancer Lett. 2012 Jul 1;320(1):96-103. (IF 7.36)
17. Wang J, Liu X, Hong Y, Wang S, Chen P, Gu A, Guo X, Zhao P. . Ibrutinib, a Bruton's tyrosine kinase inhibitor, exhibits antitumoral activity and induces autophagy in glioblastoma. J EXP CLIN CANC RES. 2017 Jul 17;36(1):96. (IF 7.068)
18. Sui X, Zhang C, Zhou J, Cao S, Xu C, Tang F, Zhi X, Chen B, Wang S, Yin L. . Resveratrol inhibits Extranodal NK/T cell lymphoma through activation of DNA damage responsepathway. J EXP CLIN CANC RES. 2017 Sep 26;36(1):133. (IF 7.068)
19. Chen DG, Zhu B, Lv SQ, Zhu H, Tang J, Huang C, Li Q, Zhou P, Wang DL, Li GH. . Inhibition of EGR1 inhibits glioma proliferation by targeting CCND1 promoter. J EXP CLIN CANC RES. 2017 Dec 15;36(1):186. (IF 7.068)
20. Zhang P, Lai ZL, Chen HF, Zhang M, Wang A, Jia T, Sun WQ, Zhu XM, Chen XF, Zhao Z, Zhang J. . Curcumin synergizes with 5-fluorouracil by impairing AMPK/ULK1-dependent autophagy, AKTactivity and enhancing apoptosis in colon cancer cells with tumor growth inhibition in xenograft mice. J EXP CLIN CANC RES. 2017 Dec 22;36(1):190. (IF 7.068)
21. Liu M, Zhou G, Song W, Li P, Liu H, Niu X, Fan Y. . Effect of nano-hydroxyapatite on the axonal guidance growth of rat cortical neurons. Nanoscale. 2012 May 21;4(10):3201-7. (IF 6.895)
22. Cai H, Yin D, Zhang L, Yang X, Xu X, Liu W, Zheng X, Zhang H, Wang J, Xu Y, Cheng D, Zheng M, Han Y, Wu M, Wang Y. . Preparation and biological evaluation of 2-amino-6-[18F]fluoro-9-(4-hydroxy-3-hydroxy-methylbutyl) purine (6-[18F]FPCV) as a novel PET probe for imaging HSV1-tk reporter gene expression. Nucl Med Biol. 2007 Aug;34(6):717-25. (IF 2.396)
23. Zhang JQ, Zhao XK, He J, Zhu L, Wang XJ. . Express and role of FGFR2 in bladder transitional cell carcinoma. 《中国医学工程》. 2007 Sep;15(9):720-5.
24. Sun YG, Wang XW, Yang SM, Zhou G, Wang WQ, Wang HB, Wang RQ, Fang DC. . Inhibition of nucleostemin upregulates CDX2 expression in HT29 cells in response to bile acid exposure: implications in the pathogenesis of Barrett's esophagus. J Gastrointest Surg. 2009 Aug;13(8):1430-9. (IF 2.573)
25. Hua W, Jiang J, Rong X, Wu R, Qiu H, Zhang Y, Chen Q. . The dual role of the cystathionine gamma-lyase/hydrogen sulfide pathway in CVB3-induced myocarditis in mice. BIOCHEM BIOPH RES CO. 2009 Oct 23;388(3):595-600. (IF 2.985)
26. Li H, Zhang L, Huang Q. . Differential expression of mitogen-activated protein kinase signaling pathway in the hippocampus of rats exposed to chronic unpredictable stress. Behav Brain Res. 2009 Dec 14;205(1):32-7. (IF 2.977)
27. Cai J, Yu C, Liu Y, Chen S, Guo Y, Yong J, Lu W, Ding M, Deng H. . Generation of homogeneous PDX1(+) pancreatic progenitors from human ES cell-derived endoderm cells. J Mol Cell Biol. 2010;2(1):50-60. (IF 4.0)
28. Hou T, Shi Y, Cheng S, Yang X, Li L, Xiao C. . Nogo-A expresses on neural stem cell surface. Int J Neurosci. 2010 Mar;120(3):201-5. (IF 2.107)
29. Li R, Zhang J, Zhang L, Cui Q, Liu H. . Angelica injection promotes peripheral nerve structure and function recovery with increasedexpressions of nerve growth factor and brain derived neurotrophic factor in diabetic rats. Curr Neurovasc Res. 2010 Aug;7(3):213-22. (IF 1.649)
30. Wang C, Xie H, Liu X, Qin Q, Wu X, Liu H, Liu C. . Role of nuclear factor-κB in volatile anaesthetic preconditioning with sevoflurane during myocardial ischaemia/reperfusion. EUR J ANAESTH. 2010 Aug;27(8):747-56. (IF 4.5)
31. Yu LN, Yu J, Zhang FJ, Yang MJ, Ding TT, Wang JK, He W, Fang T, Chen G, Yan M. . Sevoflurane postconditioning reduces myocardial reperfusion injury in rat isolated hearts viaactivation of PI3K/Akt signaling and modulation of Bcl-2 family proteins. J ZHEJIANG UNIV-SC B. 2010 Sep;11(9):661-72. (IF 2.082)
32. Wang W, Wang L, Li XX, Chen X, Zhang HY, He Y, Wang JJ, Zhao YY, Zhang BL, Xu YX. . Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcinegranulosa cells. J ZHEJIANG UNIV-SC B. 2010 Sep;11(9):719-27. (IF 2.082)
33. Wang X, Yang Q, Wang P, Luo L, Chen Z, Liao B, Li G. . Derp2-mutant gene vaccine inhibits airway inflammation and up-regulates Toll-like receptor 9 in anallergic asthmatic mouse model. ASIAN PAC J ALLERGY. 2010 Dec;28(4):287-93. (IF 1.247)
34. Wu J, Song R, Song W, Li Y, Zhang Q, Chen Y, Fu Y, Fang W, Wang J, Zhong Z, Ling H, Zhang L, Zhang F. . Chlorpromazine protects against apoptosis induced by exogenous stimuli in the developing ratbrain. PLoS One. 2011;6(7):e21966. (IF 2.74)
35. Zhang L, Jiang H, Gao X, Zou Y, Liu M, Liang Y, Yu Y, Zhu W, Chen H, Ge J. . Heat shock transcription factor-1 inhibits H2O2-induced apoptosis via down-regulation of reactive oxygen species in cardiac myocytes. Mol Cell Biochem. 2011 Jan;347(1-2):21-8. (IF 2.795)
36. He Y, Zhang H, Teng J, Huang L, Li Y, Sun C. . Involvement of calcium-sensing receptor in inhibition of lipolysis through intracellular cAMP andcalcium pathways in human adipocytes. BIOCHEM BIOPH RES CO. 2011 Jan 7;404(1):393-9. (IF 2.985)
37. Chen X, Wu X, Zhao Y, Wang G, Feng J, Li Q, Qian G. . A novel binding protein of single immunoglobulin IL-1 receptor-related molecule:Paralemmin-3. BIOCHEM BIOPH RES CO. 2011 Jan 28;404(4):1029-33. (IF 2.985)
38. Tian Y, Hu Y, Wang Z, Chen K, Zhang L, Wang L, Ren M, Huang A, Tang H. . Hepatitis B virus regulates Raf1 expression in HepG2.2.15 cells by enhancing its promoter activity. Arch Virol. 2011 May;156(5):869-74. (IF 2.243)
39. Guo L, Zhang J, Yan Q, Yin M. . Establishment and characterization of RNA-edited serotonin 2C receptor isoform cell models andalteration of amyloid precursor protein ectodomain secretion in HEK293 APPSwe cells. Hum Cell. 2011 Jun;24(2):104-11. (IF 3.463)
40. Jiang S, Zu Y, Wang Z, Zhang Y, Fu Y. . Involvement of mitochondrial permeability transition pore opening in 7-xylosyl-10-deacetylpaclitaxel-induced apoptosis. Planta Med. 2011 Jul;77(10):1005-12. (IF 2.687)
41. Ding S, Peng H, Fang HS, Zhou JL, Wang Z. . Pulsed electromagnetic fields stimulation prevents steroid-induced osteonecrosis in rats. BMC MUSCULOSKEL DIS. 2011 Sep 29;12:215. (IF 1.879)
42. Feng DD, Zhang H, Zhang P, Zheng YS, Zhang XJ, Han BW, Luo XQ, Xu L, Zhou H, Qu LH, Chen YQ. . Down-regulated miR-331-5p and miR-27a are associated with chemotherapy resistance and relapse in leukaemia. J Cell Mol Med. 2011 Oct;15(10):2164-75. (IF 4.486)
43. Liu ML, Wen JJ, Xu XF, Zhao DM. . Neurotoxic effect of the complex of the ovine prion protein (OvPrP(C)) and RNA on the cultured ratcortical neurons. Neurochem Res. 2011 Oct;36(10):1863-9. (IF 3.038)
44. Du YF, Liang L, Shi Y, Long QZ, Zeng J, Wang XY, He DL. . Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle andapoptosis of human prostate cancer cell line TSU-PR1. Genet Mol Biol. 2012 Jan;35(1):164-71. (IF 1.876)
45. Wang L, Qin Y, Tong L, Wu S, Wang Q, Jiao Q, Guo Z, Lin L, Wang R, Zhao W, Zhong Z. . MiR-342-5p suppresses coxsackievirus B3 biosynthesis by targeting the 2C-coding region. ANTIVIR RES. 2012 Feb;93(2):270-9. (IF 4.101)
46. Xu XY, Shi Y, Zhang PP, Zhang F, Shen YY, Su X, Zhao BL. . E-cadherin mediates adhesion and endocytosis of Aspergillus fumigatus blastospores in humanepithelial cells. CHINESE MED J-PEKING. 2012 Feb;125(4):617-21. (IF 1.585)
47. Deng J, Huang Q, Wang F, Liu Y, Wang Z, Wang Z, Zhang Q, Lei B, Cheng Y. . The role of caveolin-1 in blood-brain barrier disruption induced by focused ultrasound combinedwith microbubbles. J Mol Neurosci. 2012 Mar;46(3):677-87. (IF 2.678)
48. Liu S, Gao S, Wang XY, Wang DB. . Expression of miR-126 and Crk in endometriosis: miR-126 may affect the progression ofendometriosis by regulating Crk expression. Arch Gynecol Obstet. 2012 Apr;285(4):1065-72. (IF 2.283)
49. Yu Y, Mu J, Fan Z, Lei G, Yan M, Wang S, Tang C, Wang Z, Yu J, Zhang G. . Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of humanperiodontal ligament stem cells via ERK and JNK MAPK pathways. Histochem Cell Biol. 2012 Apr;137(4):513-25. (IF 3.418)
50. Yin C, Zhou S, Jiang L, Sun X. . Mechanical injured neurons stimulate astrocytes to express apolipoprotein E through ERKpathway. Neurosci Lett. 2012 Apr 25;515(1):77-81. (IF 2.274)
51. Chen X, Gao YD, Yang J. . Elevated interferon regulatory factor 4 levels in patients with allergic asthma. J Asthma. 2012 Jun;49(5):441-9. (IF 1.899)
52. Zhang D, Jin T, Xu YQ, Lu Y, Wu Q, Zhang YK, Liu J. . Diurnal-and sex-related difference of metallothionein expression in mice. J Circadian Rhythms. 2012 Jul 24;10(1):5. (IF 2.31)
53. Chen Y, Wang Z, Xie Y, Guo X, Tang X, Wang S, Yang S, Chen K, Niu Y, Ji W. . Folic acid deficiency inhibits neural rosette formation and neuronal differentiation from rhesusmonkey embryonic stem cells. J Neurosci Res. 2012 Jul;90(7):1382-91. (IF 4.699)
54. Ye XZ, Xu SL, Xin YH, Yu SC, Ping YF, Chen L, Xiao HL, Wang B, Yi L, Wang QL, Jiang XF, Yang L, Zhang P, Qian C, Cui YH, Zhang X, Bian XW. . Tumor-associated microglia/macrophages enhance the invasion of glioma stem-like cells via TGF-β1 signaling pathway. J Immunol. 2012 Jul 1;189(1):444-53. (IF 4.886)
55. Li P, Huang JJ, Ni JJ, Sun FY. . VEGF evokes reactive astroglia to convert into neuronal cells by affecting the biological functionof MeCP2 in adult rat brain after cerebral ischemia. Neurochem Int. 2012 Jul (IF 3.881)
56. Li FF, Shen J, Shen HJ, Zhang X, Cao R, Zhang Y, Qui Q, Lin XX, Xie YC, Zhang LH, Jia YL, Dong XW, Jiang JX, Bao MJ, Zhang S, Ma WJ, Wu XM, Shen H, Xie QM, Ke Y. . Shp2 Plays an Important Role in Acute Cigarette Smoke-Mediated Lung Inflammation. J Immunol. 2012 Sep 15;189(6):3159-67. (IF 4.886)
57. Lv XH, Chen JW, Zhao G, Feng ZZ, Yang DH, Sun WW, Fan JS, Zhu GH. . N-myc downstream-regulated gene 1/Cap43 may function as tumor suppressor in endometrialcancer. J CANCER RES CLIN. 2012 Oct;138(10):1703-15. (IF 3.656)
58. Chen HY, Wang JM, Wang HY, Zhang YX, Liu W, Pan L, Wang WH, Chen SF, Jin WG, Wang L. . Effect of short hairpin RNA-induced CXCR4 silence on ovarian cancer cell. Biomed Pharmacother. 2012 Oct;66(7):549-53. (IF 4.545)
59. Jia P, Xu YJ, Zhang ZL, Li K, Li B, Zhang W, Yang H. . Ferric ion could facilitate osteoclast differentiation and bone resorption through the production ofreactive oxygen species. J Orthop Res. 2012 Nov;30(11):1843-52. (IF 2.728)
60. Gao C, Wang X, Chen L, Wang JH, Gao ZT, Wang H. . Knockdown of Bcl-3 inhibits cell growth and induces DNA damage in HTLV-1-infected cells. ASIAN PAC J CANCER P . 2013;14(1):405-8. (IF 1.24)
61. Yan B, Zhou Y, Feng S, Lv C, Xiu L, Zhang Y, Shi J, Li Y, Wei P, Qin Z. . β -Elemene-Attenuated Tumor Angiogenesis by Targeting Notch-1 in Gastric Cancer Stem-Like Cells. EVID-BASED COMPL ALT. 2013;2013:268468. (IF 1.813)
62. Hu LF, Qian AQ, WangY, Di SM, Shang P. . Inhibitory Effect of Simulated Microgravity on Differentiating Preosteoblasts. Advances in Space Research. 2013 Jan 1;.51(1):107-14. (IF 1.746)
63. Wang J, Li J, Huang Y, Song X, Niu Z, Gao Z, Wang H. . Bcl-3 suppresses Tax-induced NF-κB activation through p65 nuclear translocation blockage in HTLV-1-infectedcells. Int J Oncol. 2013 Jan;42(1):269-76. (IF 3.899)
64. Tang H, Ge L, Shao W, Qiu Y, Cui D. . Effect of targeted silencing of hTERT mRNA by lentivirus-mediated siRNA on A549 lung cancer cells in vitro. Mol Biol Rep. 2013 Jan;40(1):605-16. (IF 1.402)
65. Wang P, Wang X, Yang X, Liu Z, Wu M, Li G. . Budesonide suppresses pulmonary antibacterial host defense by down-regulating cathelicidin-related antimicrobial peptide in allergic inflammation mice and in lung epithelial cells. BMC Immunol. 2013 Feb 6;14:7. (IF 2.495)
66. Li X, Dong X, Zheng S, Xiao J. . Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells. Oncol Lett. 2013 Mar;5(3):865-9. (IF 2.311)
67. Lu T, Luo Y, Sun H, Qin W, Li Y. . Electroacupuncture improves behavioral recovery and increases SCF/c-kit expression in a ratmodel of focal cerebral ischemia/reperfusion. Neurol Sci. 2013 Apr;34(4):487-95. (IF 2.415)
68. Wei Q, Shi F. . Cleavage of poly (ADP-ribose) polymerase-1 is involved in the process of porcine ovarian follicular atresia. Anim Reprod Sci. 2013 May;138(3-4):282-91. (IF 1.66)
69. Wang J, Wang X, Gao C, Song X, Niu Z, Gao Z, Qin Z, Chang J, Wang H. . The pyrimidine analog FNC inhibits cell proliferation and viral protein synthesis in HTLV-1-infected cells. Mol Med Rep. 2013 May;7(5):1656-60. (IF 2.1)
70. Wang C, Ma W, Su Y. . NF-κB pathway contributes to cadmium-induced apoptosis of porcine granulosa cells. Biol Trace Elem Res. 2013 Jun;153(1-3):403-10. (IF 2.639)
71. Wang J, Sai K, Chen FR, Chen ZP. . miR-181b modulates glioma cell sensitivity to temozolomide by targeting MEK1. CANCER CHEMOTH PHARM. 2013 Jul;72(1):147-58. (IF 2.967)
72. Yu ZY, Bai YN, Luo LX, Wu H, Zeng Y. . Expression of microRNA-150 targeting vascular endothelial growth factor-A is downregulated under hypoxiaduring liver regeneration. Mol Med Rep. 2013 Jul;8(1):287-93. (IF 2.1)
73. Zhuo C, Ji Y, Chen Z, Kitazato K, Xiang Y, Zhong M, Wang Q, Pei Y, Ju H, Wang Y. . Proteomics analysis of autophagy-deficient Atg7-/- MEFs reveals a close relationship between F-Actin and autophagy. BIOCHEM BIOPH RES CO. 2013 Aug 2;437(3):482-8. (IF 2.985)
74. Liu JH, Xu TT, Liu YJ, Zhu WY, Mao SY. . A high-grain diet causes massive disruption of ruminal epithelial tight junctions in goats. AM J PHYSIOL-REG I. 2013 Aug 1;305(3):R232-41. (IF 2.992)
75. Chen Z, Yang Y, Yang X, Zhou C, Li F, Lei P, Zhong L, Jin X, Peng G. . Immune effects of optimized DNA vaccine and protective effects in a MPTP model of Parkinson's disease. Neurol Sci. 2013 Sep;34(9):1559-70. (IF 2.415)
76. Shen X, Tang J, Hu J, Guo L, Xing Y, Xi T. . MiR-424 regulates monocytic differentiation of human leukemia U937 cells by directly targeting CDX2. Biotechnol Lett. 2013 Nov;35(11):1799-806. (IF 1.977)
77. Li WL, Chen ML, Liu SS, Li GL, Gu TY, Liang P, Qin YM, Zhan YH, Quan Y, Zhang GH. . Sweet Preference Modified by Early Experience in Mice and the Related Molecular Modulations on the PeripheralPathway. J Mol Neurosci. 2013 Nov;35(11):1799-806. (IF 2.678)
78. Li X, Wu L, Liu W, Jin Y, Chen Q, Wang L, Fan X, Li Z, Cheng Y. . A network pharmacology study of Chinese medicine QiShenYiQi to reveal its underlying multi-compound, multi-target, multi-pathway mode of action. PLoS One. 2014 May 9;9(5):e95004. (IF 2.74)
79. Li Y, Shen L, Li C, Huang J, Zhao B, Sun Y, Li S, Luo Y, Qiu HJ. . Visualization of the Npro protein in living cells using biarsenically labeling tetracysteine-tagged classicalswine fever virus. Virus Res. 2014 Aug 30;189:67-74. (IF 2.934)
80. Lu X, Chen Y, Zeng T, Chen L, Shao Q, Qin W. . Knockout of the HCC suppressor gene Lass2 downregulates the expression level of miR-694. Oncol Rep. 2014 Dec;32(6):2696-702. (IF 3.417)
81. Wang F, Lin J, Hou W, Huang MY, Sun PH, Chen WM. . 5-Benzylidene-3,4-dihalo-furan-2-one derivatives inhibit human leukemia cancer cells throughsuppression of NF-κB and GSK-3β. ANTI-CANCER AGENT ME. 2015;15(6):744-54. (IF 2.049)
82. Xu X, Mao W, Chai Y, Dai J, Chen Q, Wang L, Zhuang Q, Pan Y, Chen M, Ni G, Huang Z. . Angiogenesis Inhibitor, Endostar, Prevents Vasa Vasorum Neovascularization in a Swine AtherosclerosisModel. J Atheroscler Thromb. 2015;22(10):1100-12. (IF 3.876)
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92. Deng J, Chen S, Wang F, Zhao H, Xie Z, Xu Z, Zhang Q, Liang P, Zhai X, Cheng Y. . Effects of hnRNP A2/B1 Knockdown on Inhibition of Glioblastoma Cell Invasion, Growth and Survival. Mol Neurobiol. 2016 Mar;53(2):1132-44. (IF 4.5)
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137. Xu Y, Wang Z, Jiang X, Cui Y. . Overexpression of long noncoding RNA H19 indicates a poor prognosis for cholangiocarcinoma and promotes cell migration and invasion by affecting epithelial-mesenchymal transition. Biomed Pharmacother. 2017 Aug;92:17-23. (IF 4.545)
138. Lin J, Wang C, Zhang L, Wang T, Zhang J, Liang W, Li C, Qian G, Ouyang Y, Guo K, Zhang Y. . Rab5 Enhances Classical Swine Fever Virus Proliferation and Interacts with Viral NS4B Protein to Facilitate Formation of NS4B Related Complex. Front Microbiol. 2017 Aug 8;8:1468. (IF 4.235)
139. Sun L, Yuan Q, Xu T, Yao L, Feng J, Ma J, Wang L, Lu C, Wang D. . Pioglitazone Improves Mitochondrial Function in the Remnant Kidney and Protects against RenalFibrosis in 5/6 Nephrectomized Rats. Front Pharmacol. 2017 Aug 15;8:545. (IF 4.225)
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149. Dong XL, Wu YF, Liu TH, Wang W, Pan CX, Adur M, Zhang MJ, Pan MH, Lu C. . Bombyx mori protein BmREEPa and BmPtchd could form a complex with BmNPV envelope protein GP64. BIOCHEM BIOPH RES CO. 2017 Sep 2;490(4):1254-1259. (IF 2.985)
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152. Xu X, Lv H, Li X, Su H, Zhang X, Yang J. . Danshen attenuates cartilage injuries in osteoarthritis in vivo and in vitro by activatingJAK2/STAT3 and AKT pathways. EXP ANIM TOKYO. 2017 Oct 31. (IF 1.574)
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人S 蛋白100B (S-100B) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中S 蛋白100B (S-100B) 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 S 蛋白 100B (S-100B) 水平。用纯化的人 S 蛋白 100B (S-100B) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 S 蛋白 100
Introduction to QM/MM Simulations
Hybrid quantum mechanics/molecular mechanics (QM/MM) simulations have become a popular tool for investigating chemical reactions in condensed phases. In QM/MM methods, the region of the system in which the chemical process takes place
科研君们,当埋头实验几月找到了目标基因片段,电泳时条带却突然消失了;千辛万苦分离纯化的物质,色谱图中却意外出现杂质峰,苦苦查询,可能仍找不到问题原因。若问题只在小小的吸头上,科研君的心还能淡定吗? 吸头的问题在哪里,如何保证纯净无污染的吸头?需要考虑以下几方面因素: 一、 吸头材质 吸头市场上,原材料基本都是聚丙烯塑料(低吸附,化学惰性高,使用温度范围广,无色透明)。也许大家不知道的是,同样是聚丙烯,品质会有大的差别。高品质的吸头一般采用天然 100% 纯聚丙烯,而低廉的吸头则很多












