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文献和实验Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
0.05mol/L Tris-HCl缓冲液(pH7.19~9.10)
pH 0.2mol/L Tris(ml) 0.2mol/L HCl(ml) H2 O
2 μl 1M MgCl2 ©> 100μl H2O 4)binding rxn: components titrated & optimized by gel shiftassays 2μl probe mix Xμl extract ©>18μl NEB (see nucprp.ptc) 30' RT 5)DNAse rxn: add 2μl DNAse mix to binding rxn inc 1' RT stop w/ 100μl DNAse stop
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