Human 888MEL (BRAFV600E) mutant melanoma cell lines
浓度
~10μM
处理时间
4 days
方法
Cells are plated in quadruplicate at a density of 3,000 per well in 384-well plates in normal growth medium and allowed to adhere overnight. Compounds are added in 10 concentrations based on a 3-fold dilution series. Cell viability is measured 72h later using the CellTiter-Glo Luminescent Cell Viability Assay.