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文献和实验-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml. Autoclave. 1. Rock bottle gently to make a uniform slurry. 2. Place a disposable 3ml chromatography column into a 15 ml
Used to removed unincorporated nucleotides from labelling reactions.Prepare Sephadex G-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml. Autoclave.1. Rock bottle gently to make
Fastfilter Plasmid Maxi Kit Spin Protocol
实验步骤 Growth of bacterial culture: 1. Culture volume: Inoculate 200 ml LB/ampicillin (50 ug/ml) medium placed in a 1-4 liter culture flask with E.coli carrying desired plasmid and grow at 37°C with agitation for 12-16
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