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文献和实验Antibody Techniques Used in the Study of Anaplastic Lymphoma Kinase-Positive ALCL
of leukemia and lymphoma pathology. The use of antibodies specific for anaplastic lymphoma kinase (ALK) protein has permitted the identification of the tumor entity ALK-positive from the poorly defined morphological category of anaplastic large cell lymphoma
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
affinity) and the other is an IP kinase reaction. Substrate/Antibody, Lysate Interaction: 1. For GST c-Jun substrate interaction the quantity of protein on the beads is variable with the batch made (make fresh at least once a week). Generally
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