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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
也有类似产品。在我们这里先介绍两种目的蛋白的检测实验，we presented data on two targets: p38 mitogen activated protein kinase (MAPK) and stress-activated protein kinase/Jun-terminal kinase (SAPK/JNK)其中P38 MAPK和JNK是参与到separate MAP kinase cascade pathways and function mainly
Detection of ERK1/2 Activities Using Affinity Reagents
The three major mammalian mitogen-activated protein kinase (MAPK) subfamilies include the extracellular signal-regulated kinases (ERK1/2), the c-Jun N-terminal kinases (JNKs), and the p38 kinases. These kinases are differentially activated
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