Port-a-Patch mini全自动膜片钳

Port-a-Patch mini -更小巧而强大的全自动膜片钳

随着Port-a-Patch mini的发布，世界上最小的膜片钳装置就变得更小巧了。 基于Port-a-Patch的成功，我们通过将放大器集成到工作站中，从而进一步缩小了膜片钳系统。 Port-a-Patch mini一次支持对一个细胞进行千兆封接记录，只需简短的培训即可快速轻松地获得高质量的膜片钳数据。它不仅是功能强大的研究工具，还是用于教学目的以及细胞和离子通道快速测试的理想之选。 Port-a-Patch mini的优点包括：

• 小型且价格合理的完整膜片钳系统
• 尽量减少测试细胞和待测物的花费
• 内置放大器
• 不需要膜片钳经验
• 适合教学
• 支持电压，电流钳
• 支持全细胞，穿孔膜记录
• Nanion自产芯片，提供多种电阻规格 (可另外定制芯片规格)
• 手动，无限制加入待测物
• 高成功率（细胞系），也可成功用于原代细胞与干细胞
• 易于使用，只需要简单培训

使用Port-a-Patch mini既简单又容易-用户只需将溶液和细胞添加到一次性记录芯片上，即可使用计算机控制的气泵通过抽吸自动捕获和封接细胞。

Port-a-Patch微型系统由带内置放大器的Port-a-Patch微型记录工作站，抽吸控制器和计算机组成。
PatchControl mini：Port-a-Patch mini的直观软件
PatchControl mini是一款基于Port-a-Patch的知名PatchControl软件，并针对Port-a-Patch mini进行优化，使您能够以最简单的方式执行密封操作。配备了一组预先编写的协议，适合大多数用户场景，以获得一个完全自动化的过程，以达到整个单元配置，协议也可以更改，允许整个过程的单元捕获，密封和获得整个单元配置完全可定制。然而，因为我们知道有时你只是想自己控制事情，协议可以随时中断和手动控制吸。
PatchControl mini通过与电生理学软件WinWCP的创新沟通，读取膜电阻、全细胞电容、串联电阻等重要参数，并利用这些参数执行相应的预编程自动化步骤。吸力强度和持续时间，电压和膜标准可以设置和改变，以获得最佳参数的细胞常规使用在您的实验室。
PatchControl迷你软件可以使您的膜片钳生活更容易，从自动细胞捕获到自动压力控制，PatchControl迷你软件可以做一切!
数据与应用：

Astrocytes - Current Traces of Potassium Channels

   Port-a-Patch mini data and applications:
Native mouse Astrocytes.

Patch clamp recordings from cultured mouse primary astrocytes were made in the whole cell configuration. Currents of expressed Potassium channels were obtained by depolarizing voltage pulses from a holding potential of −80 mV. 
 

Chondrocytes - Current response to voltage ramp

   Port-a-Patch mini data and applications:
Native Bovine Chondrocytes were kindly provided by Dr. Rebecca Lewis, University of Surrey.

Patch clamp recordings from primary chondrocytes were made in the whole cell configuration. The raw current trace was obtained by using a ramp protocol starting from -100 mV to 100 mV. 

hERG - Application of Dofetilide

   Port-a-Patch mini data and applications:
Cells were kindly provided by SB Drug Discovery.

Raw data traces of hERG (expressed in HEK cells) in control solution and in the presence of increasing concentrations of Dofetilide (top). IC₅₀ concentration response curve for an average of 3 cells is shown revealing an IC₅₀ of 161 ± 12 nM.

 

 

 

NaV1.5 - Raw current traces and Current-Voltage Relationship

   Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.

The image shows currents from an example cell and the corresponding current-voltage plot for Na_V1.5 expressed in stably-transfected CHO-K1 cells.

NaV1.5 - Tetracaine concentration response curve

   Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.

The image shows the timecourse of the experiment (top) where peak amplitude is plotted against time and example traces in control conditions and different concentrations of tetracaine are also shown in the insert. The concentration reponse curve for tetracaine is shown on the bottom for an average of 6 cells. The curve is fit with a Hill equation revealing an IC₅₀ = 25.5 ±  5.7 µM (n = 6). 
 

NaV1.5-Late - Recorded from Cardiosight-S hiPSC-CMs

   Port-a-Patch mini data and applications:
Cells were kindly provided by Nexel.

NaV-Late was induced by application of ATX II in Cardiosight-S^® cells on the Port-a-Patch mini. Example traces are shown on the top with the response to the voltage ramp (CiPA protocol) shown in the inset. The timecourse of the experiment is shown on the bottom, with block by ranolazine.