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Recombinant Dengue Antigen for Rapid Test
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文献和实验loop of the polypeptide chain is predominantly responsible for the antibody binding. In the second—discontinuous epitopes—linear peptides do not bind to the antibody, but the important amino acids can be mapped by mutational analysis (10
Immunocytology using the HA epitope
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
Generation and Use of Epitope-Tagged Receptors
Epitope tagging of a receptor involves introducing a defined amino acid sequence, to which an antibody has already been produced, into the primary amino acid sequence of the receptor. The new sequence can be as short as 10–15 amino acids
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