- ￥4500
- 义翘神州
- 40592-V05H
- 北京
- 2025年08月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
999
- 英文名：
SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)
- 保质期：
12个月
- 供应商：
北京义翘神州科技股份有限公司
- 保存条件：
-20℃ to -80℃
- 规格：
100 µg
SARS-CoV-2 （2019-nCoV） 新型冠状病毒 Spike Protein 重组蛋白(RBD, mFc Tag)产品信息：
新型冠状病毒S-RBD蛋白由义翘神州自主研发，蛋白纯度经过SDS-PAGE电泳检测超过95%。新型冠状病毒S蛋白由哺乳动物HEK293细胞表达，蛋白结构更接近天然结构。经过活性检测，此新型冠状病毒S蛋白具有良好的ACE2结合活性。新型冠状病毒重组蛋白有457个氨基酸构成，预计分子量为51.5 kDa。新型冠状病毒S-RBD蛋白SDS-PAGE电泳结果如下图：
新型冠状病毒S-RBD蛋白SEC-HPLC检测>95%
新型冠状病毒S-RBD蛋白活性检测结果：
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)(40592-V05H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag) is 15-50 ng/mL.
2. Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.
3. Loaded Human ACE2 Protein (His tag) (Cat. No. 10108-H08H) on SA Biosensor, can bind SARS-CoV-2/2019-nCoV Spike/RBD Protein (RBD, mFc Tag) (Cat. No. 40592-V05H) with an affinity constant of 0.562 nM as determined by Octet RED System.
北京义翘神州科技股份有限公司（Sino Biological Inc.）创建于2007年，是一家从事生物试剂研发、生产、销售并提供技术服务的生物科技公司，主要业务包括重组蛋白、抗体、基因和培养基等产品，以及重组蛋白、抗体的开发和生物分析检测等服务。
义翘神州为全球的药品研发企业和生命科学研究机构提供高质量的生物试剂产品和高水平的技术服务，能够覆盖生命科学研究的多个领域，为分子生物学、细胞生物学、免疫学、发育生物学、干细胞研究等基础科研方向和创新药物研发提供“一站式”生物试剂产品和技术服务。客户涵盖大学、科研院所、医药研发企业等国内外各类生物研发单位。目前公司已经在美国、欧洲等地建立了子公司，在上海建立分公司，已成为生物试剂行业国内领先的科技公司之一。
凭借多年病毒蛋白研究技术优势，义翘神州仅用11天就成功表达出新冠病毒重要靶点受体结合域蛋白。为了更好的支持新冠病毒研究，义翘神州已开发生产近百种新冠病毒相关科研试剂，包括重要靶点抗原蛋白、中和抗体、抗原蛋白检测试剂盒等。义翘神州还可以提供新冠假病毒中和检测服务，可用于筛选出具有中和活性的抗体或血清，支持在细胞水平上的评估。目前，这些科研试剂已被全球数百家研究机构用于疫苗和抗体开发及基础机制研究。
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- 作者
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文献和实验该产品被引用文献
1, Xiong HL, et al. Robust neutralization assay based on SARS-CoV-2 S-protein-bearing vesicular stomatitis virus (VSV) pseudovirus and ACE2-overexpressing BHK21 cells.Emerging microbes & infections, PubMed ID: 32893735
2, Chi X, et al. Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain.Nature communications, PubMed ID: 32913273
3, Piccoli L, et al. Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.Cell, PubMed ID: 32991844
4, Huang PH, et al. Neutralizing antibody response elicited by SARS-CoV-2 receptor-binding domain.Human vaccines & immunotherapeutics, PubMed ID: 32991231
5, Zhu ZL, et al. Blocking Effect of Demethylzeylasteral on the Interaction between Human ACE2 Protein and SARS-CoV-2 RBD Protein Discovered Using SPR Technology.Molecules (Basel, Switzerland), PubMed ID: 33374387
6, Haymond A, et al. Viral Neutralization is Durable in Asymptomatic COVID-19 for at least 60 Days.The Journal of infectious diseases, PubMed ID: 33718952
7, Guo Y, et al. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes.Nature communications, PubMed ID: 33976198
8, Nie J, et al. Three epitope-distinct human antibodies from RenMab mice neutralize SARS-CoV-2 and cooperatively minimize the escape of mutants.Cell discovery, PubMed ID: 34285195
9, Schepens B, et al. An affinity-enhanced, broadly neutralizing heavy chain-only antibody protects against SARS-CoV-2 infection in animal models.Science translational medicine, PubMed ID: 34609205
10, Duan X, et al. Dual-detection fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike RBD-ACE2 blocking neutralizing antibody.Biosensors & bioelectronics, PubMed ID: 34942543
11, Bassi J, et al. Poor neutralization and rapid decay of antibodies to SARS-CoV-2 variants in vaccinated dialysis patients.PloS one, PubMed ID: 35143540
12, Walter JD, et al. Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance.EMBO reports, PubMed ID: 35253970
13, Li T, et al. Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19.Biosensors, PubMed ID: 35323418
14, Kotani N, et al. Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis.The Journal of biological chemistry, PubMed ID: 36152751
15, Li M, et al. Bergamottin and PAP-1 Induced ACE2 Degradation to Alleviate Infection of SARS-CoV-2.International journal of molecular sciences, PubMed ID: 36293419
16, Marzi R, et al. Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape.iScience, PubMed ID: 36507220
17, Addetia A, et al. Therapeutic and vaccine-induced cross-reactive antibodies with effector function against emerging Omicron variants.bioRxiv : the preprint server for biology, PubMed ID: 36711984
18, Zheng J, et al. Deciphering intercellular signaling complexes by interaction-guided chemical proteomics.Nature communications, PubMed ID: 37438365
19, Di Primio C, et al. Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons.PNAS nexus, PubMed ID: 37731949
20, Quaranta P, et al. SARS-CoV-2 Infection Alters the Phenotype and Gene Expression of Adipocytes.International journal of molecular sciences, PubMed ID: 38396763
21, Chen X, et al. An oncolytic virus delivering tumor-irrelevant bystander T cell epitopes induces anti-tumor immunity and potentiates cancer immunotherapy.Nature cancer, PubMed ID: 38609488
22, Luo Y, et al. SARS-Cov-2 spike induces intestinal barrier dysfunction through the interaction between CEACAM5 and Galectin-9.Frontiers in immunology, PubMed ID: 38686388
2, Chi X, et al. Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain.Nature communications, PubMed ID: 32913273
3, Piccoli L, et al. Mapping Neutralizing and Immunodominant Sites on the SARS-CoV-2 Spike Receptor-Binding Domain by Structure-Guided High-Resolution Serology.Cell, PubMed ID: 32991844
4, Huang PH, et al. Neutralizing antibody response elicited by SARS-CoV-2 receptor-binding domain.Human vaccines & immunotherapeutics, PubMed ID: 32991231
5, Zhu ZL, et al. Blocking Effect of Demethylzeylasteral on the Interaction between Human ACE2 Protein and SARS-CoV-2 RBD Protein Discovered Using SPR Technology.Molecules (Basel, Switzerland), PubMed ID: 33374387
6, Haymond A, et al. Viral Neutralization is Durable in Asymptomatic COVID-19 for at least 60 Days.The Journal of infectious diseases, PubMed ID: 33718952
7, Guo Y, et al. A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes.Nature communications, PubMed ID: 33976198
8, Nie J, et al. Three epitope-distinct human antibodies from RenMab mice neutralize SARS-CoV-2 and cooperatively minimize the escape of mutants.Cell discovery, PubMed ID: 34285195
9, Schepens B, et al. An affinity-enhanced, broadly neutralizing heavy chain-only antibody protects against SARS-CoV-2 infection in animal models.Science translational medicine, PubMed ID: 34609205
10, Duan X, et al. Dual-detection fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike RBD-ACE2 blocking neutralizing antibody.Biosensors & bioelectronics, PubMed ID: 34942543
11, Bassi J, et al. Poor neutralization and rapid decay of antibodies to SARS-CoV-2 variants in vaccinated dialysis patients.PloS one, PubMed ID: 35143540
12, Walter JD, et al. Biparatopic sybodies neutralize SARS-CoV-2 variants of concern and mitigate drug resistance.EMBO reports, PubMed ID: 35253970
13, Li T, et al. Highly Sensitive Immunoresistive Sensor for Point-Of-Care Screening for COVID-19.Biosensors, PubMed ID: 35323418
14, Kotani N, et al. Host cell membrane proteins located near SARS-CoV-2 spike protein attachment sites are identified using proximity labeling and proteomic analysis.The Journal of biological chemistry, PubMed ID: 36152751
15, Li M, et al. Bergamottin and PAP-1 Induced ACE2 Degradation to Alleviate Infection of SARS-CoV-2.International journal of molecular sciences, PubMed ID: 36293419
16, Marzi R, et al. Maturation of SARS-CoV-2 Spike-specific memory B cells drives resilience to viral escape.iScience, PubMed ID: 36507220
17, Addetia A, et al. Therapeutic and vaccine-induced cross-reactive antibodies with effector function against emerging Omicron variants.bioRxiv : the preprint server for biology, PubMed ID: 36711984
18, Zheng J, et al. Deciphering intercellular signaling complexes by interaction-guided chemical proteomics.Nature communications, PubMed ID: 37438365
19, Di Primio C, et al. Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons.PNAS nexus, PubMed ID: 37731949
20, Quaranta P, et al. SARS-CoV-2 Infection Alters the Phenotype and Gene Expression of Adipocytes.International journal of molecular sciences, PubMed ID: 38396763
21, Chen X, et al. An oncolytic virus delivering tumor-irrelevant bystander T cell epitopes induces anti-tumor immunity and potentiates cancer immunotherapy.Nature cancer, PubMed ID: 38609488
22, Luo Y, et al. SARS-Cov-2 spike induces intestinal barrier dysfunction through the interaction between CEACAM5 and Galectin-9.Frontiers in immunology, PubMed ID: 38686388
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新型冠状病毒S蛋白|2019-nCoV Spike重组蛋白|SARS-COV-2 S protein
￥4500
