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文献和实验Partial proteolysis with Staph. aureus V8 protease
. If you have too much, the V8 won't work. The molarity of neat mercaptoethanol is approximately 14. 1 microliter of ME per 14 ml of solution gives a 1 mmolar solution. Enzyme stock solution: 500 micrograms/ml (or 500 nanograms/microliter) V8-protease in 125
(8 –11 ). The substrate specificity of the enzyme is (M,I,L)XGG-X or (M,I,L)XGX-G (12 ). Alkylating agents and E64 inhibit the protease and virus infection as expected (13 ). Specific inhibitors are not yet available. To date, 17 protease genes
as a blood anti-coagulant, and in the prevention of proteinaceous hazes in chill-proof beers! The assay Protease enzymes are added to a milky colloidal suspension of egg albumen. As the protease enzymes digest the suspended
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