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DEPC即diethypyrocarbonate,中文名为焦碳酸二乙酯。分子式为C6H10O5,分子量为162.14,纯度>99%。本DEPC为液体,密度为1.12g/ml。10g DEPC的体积约为9ml。
通常用于去除RNase。
包装清单:
| 产品编号 | 产品名称 | 包装 |
| ST036 | DEPC | 10g |
| - | 说明书 | 1份 |
保存条件:
4℃避光保存。
注意事项:
本产品对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
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文献和实验1. Li J, Wang W, Sun D, Chen J, Zhang P, Zhang J, Min Q and Zhu J. . Aptamer-Functionalized Silver Nanoclusters-Mediated Cell Type-Specific siRNA Delivery and Tracking. CHEM SCI. 2013;9:3514-21.(IF 9.346)
2. Yin J, Wang Y, Zhou B, Xiao X, Xue J, Wang J, Wang Y, Qian Q. . A wireless magnetoelastic sensor for uranyl using DNAzyme-graphene oxide and gold nanoparticles-based amplification. SENSOR ACTUAT B-CHEM. 2013 Nov;188:147-55.(IF 7.1)
3. Cai X, Bao L, Ren J, Li Y, Zhang Z. . Grape seed procyanidin B2 protects podocytes from high glucose-induced mitochondrial dysfunction andapoptosis via the AMPK-SIRT1-PGC-1α axis in vitro. Food Funct. 2016 Feb;7(2):805-15.(IF 4.171)
4. Zhang X, Ding B, Wu H, Wang J, Yang H. . A Label-free "Turn-on" Fluorescence Method for Detecting Mercury Ion in Aqueous SolutionBased on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA. Anal Sci. 2017;33(2):165-169.(IF 2.049)
5. Liu L, Huang R, Yang R, Wei X. . OCT4B1 Regulates the Cellular Stress Response of Human Dental Pulp Cells with Inflammation. Biomed Res Int. 2017;2017:2756891.(IF 2.276)
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7. Hu GD, Wang CX, Wang HY, Wang YQ, Hu S, Cao ZW, Min B, Li L, Tian XF, Hu HB . Long noncoding RNA CCAT2 functions as a competitive endogenous RNA to regulate FOXC1 expression by sponging miR-23b-5p in lung adenocarcinoma. J Cell Biochem. 2018 Dec 12(IF 4.237)
8. Ma XR, Xu YL, Qian J, Wang Y . Long non-coding RNA SNHG15 accelerates the progression of non-small cell lung cancer by absorbing miR-211-3p. EUR REV MED PHARMACO. 2019 Feb23(4):1536-1544.(IF 3.024)
9. Wang C, Li S, Shen C, Shan J, Fan Y . Expression and significance of phosphodiesterase 4B gene in peripheral blood of patients with oral lichen planus. Int J Dermatol. 2019 Mar58(3):302-310.(IF 2.067)
10. Ding F, You T, Hou XD, Yi K, Liu XG, Zhang P, Wang XK . MiR-21 regulates pulmonary hypertension in rats via TGF-β1/Smad2 signaling pathway. EUR REV MED PHARMACO. 2019 May23(9):3984-3992.(IF 3.024)
11. Yao N, Sun JQ, Yu L, Ma L, Guo BQ . LINC00968 accelerates the progression of epithelial ovarian cancer via mediating the cell cycle progression. EUR REV MED PHARMACO. 2019 Jun23(11):4642-4649.(IF 3.024)
12. Liu G, Jiang Z, Qiao M, Wang F . Lnc-GIHCG promotes cell proliferation and migration in gastric cancer through miR- 1281 adsorption. Mol Genet Genomic Med. 2019 Jun7(6):e711.
13. Zhou F, Fu WD, Chen L . MiRNA-182 regulates the cardiomyocyte apoptosis in heart failure. EUR REV MED PHARMACO. 2019 Jun23(11):4917-4923.(IF 3.024)
14. Sun N, Wang XQ . Correlation between COX-2 gene polymorphism and susceptibility to nasopharyngeal carcinoma. EUR REV MED PHARMACO. 2019 Jul23(13):5770-5778.(IF 3.024)
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Procurement 100ml DEPC from Sigma (D5758) [1] - �33/$292 (as of 2009-01) 100ml DEPC from MPI (150902) [2] - �13 (as of 2009-01) DEPC-treatment of solutions add 0.05-0.1% (v/v) DEPC to solution/water (More DEPC
云向 大家好,我在提RNA ,但在配DEPC水时,和以往有点小不同,以前DEPC都是过夜才容的,有时甚至过夜也不容,但这次我刚加进去,大概等了一个多点就融了,中间我摇晃了三四次,我放在37度恒温箱中了,我很奇怪,这次为什么容那么快,会不会有问题,对了,我是在温水中假的depc水,大家谁还遇到过这种现象,不知道影不影响RNA的提取? 外源RNA酶清除剂 没问题,本来DEPC就很容易溶于水。
DEPC有致癌性,不能处理Tris溶液等这些事项大家都知道,所以不谈。谈一些实验室中的操作误区。 误区一:重复使用含DEPC的水处理枪头、离心管等。由于DEPC较贵,而且书上说DEPC要用高压灭菌处理去除,于是有些人就萌发了重复使用DEPC的想法,即对含有DEPC的水不进行高压灭菌,从而重复使用含DEPC的水处理枪头、离心管等。节约本是好事,但这样的节约是无效的。为什么不能重复使用含DEPC的水,因为DEPC在水溶液中不稳定,极易分解,DEPC在pH6.0和pH7.0的PBS
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