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Tubulin beta-III Recombinant R

abbit mAb(bsm-52323R)-50ul/100ul/25ul
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  • ￥800 - 2500
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  • bsm-52323R
  • 2025年10月16日
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      50ul/100ul/25ul

    规格：50ul产品价格：￥1400.0
    规格：100ul产品价格：￥2500.0
    规格：25ul产品价格：￥800.0
    产品编号bsm-52323R
    英文名称Tubulin beta-III Recombinant Rabbit mAb
    中文名称微管蛋白β3重组兔单抗
    英文别名Neuron specific beta III Tubulin; beta 4; MC1R; TBB3_HUMAN; TUBB 3; TUBB 4; TUBB3; TUBB4; Tubulin beta 3 chain; Tubulin beta 4; Tubulin beta III; Tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-III; beta tubulin III; Neuron specific beta III Tubulin.
    产品应用WB=1:1000-10000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, cerebellum, Cerebrum, Cerebrum, Cerebrum, Cerebrum, Human left parietal lobe, Human left parietal lobe, Human left parietal lobe, Human left parietal lobe
    抗体来源Rabbit
    免疫原A synthesized peptide derived from human beta III Tubulin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Recombinant
    理论分子量50 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Neuroscience > Cell Adhesion Proteins > Cytoskeletal Proteins > Microtubules

    Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microtubules > Tubulin

    Tags & Cell Markers > Cell Type Markers > Neuroscience Markers > Neuronal

    亚基Dimer of alpha and beta chains.
    亚细胞定位Cytoplasm, cytoskeleton.
    组织特异性Expression is primarily restricted to centraland peripheral nervous system. Greatly increased expression in mostcancerous tissues.
    翻译后修饰Some glutamate residues at the C-terminus arepolyglutamylated. This modification occurs exclusively on glutamateresidues and results in polyglutamate chains on the gamma-carboxylgroup. Also monoglycylated but not polyglycylated due to theabsence of functional TTLL10 in human. Monoglycylation is mainlylimited to tubulin incorporated into axonemes (cilia and flagella)whereas glutamylation is prevalent in neuronal cells, centrioles,axonemes, and the mitotic spindle. Both modifications can coexiston the same protein on adjacent residues, and lowering glycylationlevels increases polyglutamylation, and reciprocally. The precisefunction of such modifications is still unclear but they regulatethe assembly and dynamics of axonemal microtubules (Probable).
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, frommetaphase to telophase, but not in interphase. This phosphorylationinhibits tubulin incorporation into microtubules.
    相似性Belongs to the tubulin family.
    功能Tubulin is the major constituent of microtubules. Itbinds two moles of GTP, one at an exchangeable site on the betachain and one at a non-exchangeable site on the alpha chain. TUBB3plays a critical role in proper axon guidance and mantainance.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Neuronal MarkerBeta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.

     

    应用推荐稀释比例
    {WB}{1:1000-10000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {ICC/IF}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1:50-200}

     

    产品细节图片1
    25 ug total protein per lane of various lysates (see on figure) probed with Tubulin beta-III monoclonal antibody, unconjugated (bsm-52323R) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded Human Thyroid Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Tubulin beta-III Monoclonal Antibody, Unconjugated(bsm-52323R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

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      [IF={{ 6.064 }}] {Le Wang. et al. Enteric nervous system damage caused by abnormal intestinal butyrate metabolism may lead to functional constipation. FRONT MICROBIOL. 2023; 14: 1117905} {ICC} {Mouse}

      [IF={{ 5.195 }}] {Lu Hong. et al. Investigation of Naoluoxintong on the neural stem cells by facilitating proliferation and differentiation in vitro and on protecting neurons by up-regulating the expression of nestin in MCAO rats. J ETHNOPHARMACOL. 2022 Sep;:115684} {WB} {Rat}

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      研选
      Tubulin beta-III Recombinant Rabbit mAb(bsm-52323R)-50ul/100ul/25ul
      ￥800 - 2500