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RPS6 (N-term) Mouse mAb(bsm-51

406M)-50ul/100ul
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  • ￥1580 - 2500
  • Bioss已认证
  • bsm-51406M
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul

    规格：50ul产品价格：￥1580.0
    规格：100ul产品价格：￥2500.0
    产品编号bsm-51406M
    英文名称RPS6 (N-term) Mouse mAb
    中文名称S6核糖体蛋白单克隆抗体
    英文别名40S ribosomal protein S6; Air8; DS6; Hemocytes enlarged; Hen; L(1)AIR8; Lethal(1) haemocytes enlarged; Lethal aberrant immune response 8; Lethal(1)abberant immune response[8]; phosphoprotein NP33; Pp30; Ribosomal protein S6; RP S6; RPS 6; RpS6; RS6; RS6_HUMAN; S6; S6 Ribosomal Protein.
    产品应用WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat, Cerebrum, Cerebrum, Cerebrum, Cerebrum, cerebellum, cerebellum, cerebellum, cerebellum, human lung carcinoma, human lung carcinoma, human lung carcinoma, human lung carcinoma, human cervical carcinoma, human cervical carcinoma, human cervical carcinoma, human cervical carcinoma, human colon carcinoma, human colon carcinoma, human colon carcinoma, human colon carcinoma, Cerebrum, Cerebrum, Cerebrum, cerebellum, cerebellum, cerebellum
    抗体来源Mouse
    免疫原Recombinant human RPS6
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein G
    克隆类型Monoclonal
    理论分子量29 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Ribosome

    翻译后修饰Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphrylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
    相似性Belongs to the ribosomal protein S6e family.
    功能May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:50-200}
    {IHC-F}{1:50-200}
    {IF}{1:50-200}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (RPS6 (N-term)) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024)instructionsand DAB staining.
    产品细节图片4
    25 ug total protein per lane of various lysates (see on figure) probed with RPS6 (N-term) monoclonal antibody, unconjugated (bsm-51406M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片9
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片10
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片11
    P‌‌araformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片12
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片13
    P‌‌araformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
    产品细节图片14
    P‌‌araformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with RPS6 (N-term) Monoclonal Antibody, Unconjugated (bsm-51406M) at 1:200 overnight at 4°C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

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    该产品被引用文献

    [IF={{ 4.4 }}] {Chen Shipeng. et al. Bulk RNA-seq conjoined with ScRNA-seq analysis reveals the molecular characteristics of nucleus pulposus cell ferroptosis in rat aging intervertebral discs. ARTHRITIS RES THER. 2025 Dec;27(1):1-20} {IF} {Rat}

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    文献支持
    RPS6 (N-term) Mouse mAb(bsm-51406M)-50ul/100ul
    ￥1580 - 2500