Tris-Triton-NaCl buffer (pH9.0

Preparation of Microinjection Buffer

Buffer composition is 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 30 microM spermine, 70 microM spermidine, 100 mM NaCl. This buffer is more likely to produce transgenic mice with intact, unfragmented DNA molecules. See the following references: Schedl

Restriction Enzyme Buffer

: 6.61 g Tris-HCl + 0.8 g Tris-Base (pH 7.4 at 25℃)C: 1 M MgCl2 10 ml: 2.03 gD: 3 M NaCl 20 ml: 3.50 gE: 2M KCl 10 ml: 1.48 gBan I: 0.3ml B + 0.3ml C + 0.10ml D + 0.1ml BSA + 21µl bME + 4.18ml H2OBgl II: 0.5ml B + 0.5ml C + 1.67ml D + 0.1ml BSA + 35µl bME

『原创』大家在做蛋白时的buffer配方（附件）

buffer  2.5% Triton X-100   Protease analysis; renatures enzymes after electrophoresis 10x zymogram development buffer  50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM CaCl2, 0.02% Brij-35  Protease analysis; activates enzymes after electrophoresis Premixed