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- D10376
- 2025年10月17日
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| 产品编号 | D10376 |
| 英文名称 | Lysozyme from chicken egg white |
| 中文名称 | 溶菌酶 (≥40KU/mg, 进口) |
| 英文别名 | Mucopeptide N-acetylmuramoylhydrolase, Muramidase; lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein; |
| 中文别名 | 溶解酵素; 溶菌酶 来源于鸡蛋白; 胞壁质酶; 粘肽 N-乙酰胞壁质水解酶; |
| 保存条件 | Store at -20℃. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CAS号：12650-88-3分子量：single-chain 14.3kDaMDL：MFCD00131557级别/活性：BR,≥40,000 units/mg protein 纯度：≥90% (HPLC)外观/性状：lyophilized powder酶活性定义：One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).Biochem/physiol Actions:Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).L6876 (Sigma-Aldrich) |
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文献和实验PURPOSE : The objective of this experiment is to study the effect of SU5402, a FGFR inhibitor, on early chicken development and the subsequent effects on shh expression. INTRODUCTION The development of a fertilized egg into a multicellular
Observation of living and plastic-embedded chick embryos
is that the embryo is relatively accessible and can be operated on or treated with teratogens (substances that perturb development) while still in ovo(in the egg). Finally, fertilized chicken eggs are available year round and can be "held" at 10-14ºC
Sauer:Lysing E. coli with Lysozymes
. Hen Egg White Lysozyme I prepare the lysozyme fresh each time from lyophilized powder. Pre-warm the bottle to prevent moisture from condensing in the bottle when it''s opened. Pure B-Per and most lysis buffers don''t seem to allow
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