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- CELL BIOLABS
- MET-5029
- 2025年12月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Upon receipt, store the Pyruvate Standard and 10X Assay Buffer at 4°C. Store all other components at -20ºC. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
- 库存：
100
- 规格：
100 assays
Blue Ice
存储温度：
Upon receipt, store the Pyruvate Standard and 10X Assay Buffer at 4°C. Store all other components at -20ºC. The Fluorometric Probe is light sensitive and must be stored accordingly. Avoid multiple freeze/thaw cycles.
检测方法：
Fluorometric
类别：
Kit
产品分类：
Metabolism Research->Cellular Metabolism
产品描述：
Our Pyruvate Assay Kit measures pyruvate in biological samples. First, pyruvate is oxidized by pyruvate oxidase, producing hydrogen peroxide. The hydrogen peroxide is then detected at ex. 530-570 nm/em. 590-600 nm using a specific fluorometric probe. Pyruvate levels in unknown samples are determined based on the provided pyruvate standard curve.
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文献和实验1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
相关专题 在蛋白质定量试验中，最常用的方法是BCA法蛋白定量 。那么为什么我们要选择用BCA法?而且常常使用到BCA蛋白定量试剂盒（BCA Protein Assay Kit）。因为BCA法蛋白定量具有很多优点，如1.实验操作十分简单方便;2.实验耗时短，快速;3.试剂灵敏度高，实验结果准确;4.所用的试剂稳定性好，而且经济实用，抗干扰能力强等。 实验原理： BCA(bicinchoninic acid)法蛋白浓度定量
method. The Active Motif ChIP-IT Kit comes with all the reagents needed for 25 reactions of the ChIP assay. In addition, it provides a detailed manual which saves time for protocol optimization. The protocol in the manual is easy to follow
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