- $7110
- CELL BIOLABS
- MET-5019
- 2025年12月12日
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- 详细信息
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- 技术资料
- 保存条件：
Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
- 库存：
100
- 规格：
100 assays
Dry Ice
存储温度：
Store the entire kit at -80ºC. Avoid multiple freeze/thaws by aliquoting. The Fluorometric Probe is light sensitive and should be maintained in amber tubes.
检测方法：
Fluorometric
类别：
Kit
产品分类：
Metabolism Research->Lipoprotein Metabolism->Phospholipid Assays
产品描述：
Our Total Phosphatidic Acid Assay Kit measures total phosphatidic acid content (PA and LPA) in cell lysate samples by a coupled enzymatic reaction system. First, lipase is used to hydrolyze phosphatidic acid to glycerol-3-phosphate. Next, the glycerol-3-phosphate product is oxidized by glycerol-3-phosphate oxidase (GPO), producing hydrogen peroxide. The hydrogen peroxide released from this reaction reacts specifically with the kit’s Fluorometric Probe and is detected at ex. 530-560 nm/em. 585-595 nm. Phosphatidic Acid levels in unknown sampled are determined based on the provided phosphatidic acid standard curve.
联系方式：
生物商城：http://www.annoron.com
办公电话：010-56256916
官方售后：400-965-8633
企业 QQ：3212874516、3198592576
地 址：北京经济技术开发区永昌中路4号院4号楼3层
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文献和实验Assaying Plant Phosphatidic Acid Phosphatase Activity
Phosphatidic acid phosphatase (PAP; EC 3.1.3.4) catalyzes the dephosphorylation of phosphatidic acid (PA) to produce diacylglycerol (DAG) and inorganic phosphate. In seed plants, PA plays pivotal roles both as a precursor to membrane lipids
Distinguishing Phosphatidic Acid Pools from De Novo Synthesis, PLD, and DGK
In plants, phosphatidic acid (PA) functions as a metabolic precursor in the biosynthesis of glycerolipids, but it also acts as a key signaling lipid in the response to environmental stress conditions (Testerink and Munnik, J Exp Bot 62:2349–
Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
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