- $7740
- CELL BIOLABS
- MET-5016
- 2025年12月12日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
Upon receipt, store the Sirius Red Reagent at room temperature and store the rest of the kit at 4ºC.
- 库存：
100
- 规格：
96 assays
Room Temp
存储温度：
Upon receipt, store the Sirius Red Reagent at room temperature and store the rest of the kit at 4ºC.
检测方法：
Colorimetric
类别：
Kit
产品分类：
Metabolism Research->Cellular Metabolism
产品描述：
Our Soluble Collagen Assay Kit detects soluble collagen in cell and tissue lysates. This assay uses a Sirius Red Reagent to stain the triple helix structure of collagen that has been dried onto the wells of a 96-well plate. The collagen is washed with an Acidic Reagent and the stain is eluted with a Basic Reagent, which is then transferred to a new plate and read colorimetrically between 540nm-560nm. The amount of soluble collagen in samples is determined based on a provided collagen standard curve.
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文献和实验(5 ). To overcome these drawbacks, reconstituted and hence more homogeneous ECMs were developed, and proposed as substrata to test invasiveness. Matrigel (6 ) (as described in Chapter 7 by Hall and Brooks), and employed also in the assay described in Chapter
Collagen Gel Contraction Assay
of a complex infrastructure that includes collagen and other molecules. The manner in which these two components interact is not certain, but the use of recent model systems has provided insights into these processes. The collagen lattice contraction assay
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
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