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- 2025年07月10日
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文献和实验Analysis of Rho GTPase Activation in Saccharomyces cerevisiae
of Rho proteins have become important molecular tools to decipher upstream signals and mechanisms that regulate activation and de-activation. These assays make use of Rho activation probes constructed from Rho-binding domains of downstream effectors
Affinity-Based Assay of Rho Guanosine Triphosphatase Activation
The recognition that Rho guanosine triphosphatases (GTPases) (Rho, Rac, and Cdc42) play important regulatory roles in many areas of cell biology has made the ability to measure their activity in cells an important biological tool. Because Rho
In vitro Reconstitution of Activation of PLC by Ras and Rho GTPases
discovered isoform that has been shown to be activated by members of the Ras and Rho families of guanosine trisphosphatases (GTPases) as well as subunits of heterotrimeric G-proteins. We describe a method for expressing a truncated PLCε variant as an MBP
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