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- Invitrogen™ eBioscience™
- Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- 2025年07月31日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- 英文名：
Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- CAS号：
Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- 保质期：
Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- 供应商：
上海辅泽商贸有限公司
- 保存条件：
Invitrogen™ eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) 00-4956-58
- 规格：
500 mL
货号： 00-4956-58
| 货号 | 单位规格 | 单价 (CNY) | 数量 | |
|---|---|---|---|---|
| 00-4956-58 | 500 mL | 2,311.00 |
规格
| Form: | Liquid |
|---|---|
| Product Line: | eBioscience™ |
| Product Size: | 500 mL |
| Reagent Type: | Solution |
| Validated Application: | Immunohistochemistry (IHC) |
描述
The IHC Antigen Retrieval Solution- High pH (10X) is designed for use during the heat induced epitope retrieval (HIER) step prior to immunohistochemistry on formalin-fixed paraffin embedded tissue sections. The use of this buffer in combination with heat (often by microwave, water bath, or pressure cooker) has been shown to restore the antigenicity of proteins modified during the formalin fixation of tissue. This buffer is supplied as a 10X stock solution. Dilution of this buffer to 1X using reagent grade water results in a 10 mM Tris, 1 mM EDTA solution, pH 9.0.Reported Application
Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections
For Research Use Only. Not for use in diagnostic procedures.
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文献和实验, like CANDOR’s PlateBlock™, are a possible solution to the discussed false results. Problems of blocking reagents Efficient surface blocking is an important and complex topic to be solved. Yet, many assays employ poorly defined blocking reagents
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
of your primary antibody. 1) Heat Induced Epitope Retrieval Protocol 2) Based on the reagent that you are using, dilute the reagent to 1X as follows: To 25 ml 20×Citrate Buffer, pH 6.0 solution, add 475 ml distilled water
) at the final concentration of 1 tablet/50ml): PBS Buffer A: 10 mM Hepes, pH 7.9, 10mM KCl, 1.5mM MgCl2, 0.5mM DTT S1 solution : 0.25 M Sucrose, 10 mM MgCl2 S2 solution: 0.35 M Sucrose, 0.5 mM MgCl2 S3 solution: 0.88 M Sucrose, 0.5 mM MgCl2
