- $220
- Leading Biology
- MQ002
- 2025年11月17日
- PCR, hot start PCR, qPCR, genome PCR, TA cloning, primer extension, DNA markers
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- 文献和实验
- 技术资料
- 保存条件：
-20°C for long-term storage( up to 3 year)
- 库存：
100
- 应用范围：
PCR, hot start PCR, qPCR, genome PCR, TA cloning, primer extension, DNA markers
- 浓度：
5 U/μL
- 规格：
50 μL
DNA Polymerase 产品概述：
This product is a recombinant Taq DNA polymerase modified by antibody, suitable for Hot Start PCR.At room temperature, the activity of Taq DNA polymerase is blocked by antibodies, thus inhibiting the non-specific amplification caused by the non-specific annealing of primers or the dimer of primers at room temperature.When the amplification reaction system was heated to 95°C and heated for 2min, the polymerase activity was restored due to the denaturation of Taq enzyme antibody. Therefore, no special deactivation treatment was required, and it could be used under conventional PCR reaction conditions. 活性：
One unit is the amount of enzyme that will incorporate10 nmol of dNTP into acid-insoluble products in 30 minutes at 74°C with activated salmon sperm DNA as the template-primer. 联系方式：
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文献和实验Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed
for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended
The Polymerase Chain Reaction (PCR)
• Template DNA • Reaction buffer (Tris, ammonium ions(and/or potassium ions), magnesium ions,bovine serum albumin) • Nucleotides (dNTPs) • Primers • DNA polymerase (usually Taq) PCR In Detail • Denature, anneal, extend and repeat the cycle 30 to 35
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