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- Lifeline Cell Technology
- 美国
- FC-0064
- 2025年12月30日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Human Epidermal Keratinocytes, 10-Donor Pool
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
培养试剂 DermaLife K培养基
Lifeline® normal Human Epidermal Keratinocytes (HEK), when grown in Lifeline® DermaLife K Medium, provide an ideal serum-free culture model, for the study of wound healing, toxicology or epithelial biology.
When in need of multiple keratinocyte cell lots, our Epidermal Keratinocyte 10-donor pool offers a wide range of donor demographics for research at an economical price.
Lifeline® Epidermal Keratinocytes can be grown without serum, phenol red or antimicrobials when cultured in DermaLife medium.
All Lifeline® epidermal keratinocytes are quality tested in DermaLife K Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium.
Our HEK need not be exposed to antimicrobials or phenol red when cultured in DermaLife medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental result.
- Epidermal Keratinocytes can be grown without serum, phenol red or antimicrobials when cultured in DermaLife medium.
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文献和实验In Vivo Labeling and Analysis of Epidermal Stem Cells
To identify slowly cycling (label-retaining) keratinocytes in the cutaneous epithelium of mice, newborn female pups were injected with [3 H]-thymidine twice daily for 3 d beginning the third day after birth. At 8–10 wk of age, skin
Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes
, but as much an increment of for example the sensitivity of existing methods. The in vitro generation of antibodies using phage display is one such technique, which continuously has been developed since its introduction more than 10 yr ago. As a result, selection of phage
Objectives for dermal-epidermal skin substitutes for treatment of acute and chronic wounds include, but are not limited to: increased availability; stimulation of wound healing by transplantation of parenchymal cells; regulation of wound
