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- Lifeline Cell Technology
- 美国
- FC-0037
- 2026年01月18日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Human Dermal Fibroblasts – Neonatal, Xeno-Free, Primary
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
相关产品： 培养试剂 成纤维细胞培养基
Lifeline® Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free (HDFn-XF), when grown in FibroLife® Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human pluripotent stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Human Dermal Fibroblasts-Neonatal, passage 2, 5 days after inoculation with 2000 cells/cm2 (100X)
HDFn-XF are cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HDFn-XF are quality tested in FibroLife® Xeno-Free Complete Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media containing non-human proteins or supplements.
Lifeline® HDFn-XF are not exposed to antimicrobials or phenol red when cultured in FibroLife® Xeno-Free Complete Medium, an advantage since these supplements can cause cell stress and ” masking effects” that may negatively impact experimental results.
500,000 cells per vial
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
. i. Use the MEF culture vessels within 3–4 days after plating. 2. Reprogramming Fibroblasts 1) Day –2: Prepare the cells for transduction a. 2 days before transduction, plate human neonatal foreskin fibroblast
cells may include normal, nontransformed populations isolated for primary culture, or genetically modified cells to deliver specific gene products of therapeutic interest (8 –10 ). Nontransformed cells may include autologous, allogeneic, or chimeric
Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes
., and Tsien, R.Y. 1989. Fluorescence ratio imaging of cytosolic free Na+ in individual fibroblasts and lymphocytes. J. Biol. Chem. 264: 19458‐19467. Haugland, R.P. 1993
