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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Human Lung Microvascular Endothelial Cells
- 细胞类型:
人正常原代细胞
- 物种来源:
人源
- 器官来源:
人源
- 运输方式:
干冰或液氮
- 年限:
液氮储存10年以上
- 生长状态:
冻存
- 规格:
500,000 cells/vial
Lifeline® normal Human Lung Microvascular Endothelial Cells, when grown in VascuLife® Medium, provide an ideal low-serum culture model, for the study of angiogenesis, atherosclerosis or vascular biology.
To support cell proliferation, Lifeline® recommends using VascuLife® VEGF-Mv (LL-0005) Medium.
Lifeline® Human Lung Microvascular Endothelial Cells are cryopreserved at the earliest possible passage to ensure the highest viability, purity, and plating efficiency. Our Human Lung Microvascular Endothelial Cells are quality tested to ensure optimal growth and morphology over a period of at least 15 population doublings.
Human Lung Microvascular Endothelial Cells are not exposed to antimicrobials or phenol red when cultured in VascuLife® Media. Lifeline offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.
A vial of Gentamicin and Amphotericin B (GA; LS-1104) is provided with the purchase of VascuLife® VEGF-Mv (LL-0005) Medium Complete Kits for your convenience. The use of GA is recommended to inhibit potential fungal or bacterial contamination of eukaryotic cell cultures. Phenol Red (LS-1009) may be purchased, but is not required.
Lifeline® manufactures products using the highest quality raw materials and incorporates extensive ISO-style quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.
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文献和实验实验材料: 1. 手术切除的正常肺组织 2. 胰蛋白酶/EDTA液:0.05%胰蛋白酶,0.5mmol/L EDTA 3. 6孔培养板:用多聚赖氨酸包被 4. 不含Ca2+ 和Mg2+ 的1×PBS(pH=7.4),添加200000IU/L青霉素、200mg/L链霉素和200000U/L庆大霉素,pH7.4 5. 手术刀、解剖剪、解剖镊、眼科剪,眼科镊 6. 离心管(15ml、50ml) 实验方法: 1. 将肺组织至于无菌的培养皿中用含双抗的1×PBS
thx221 有没有哪位大侠做内皮细胞方面的信号转导的???帮忙给提供几个通路做参考!或者给我点相关的最新研究文献!我实在是找不到这方面的最新研究进展,大家帮个忙吧!先谢谢了!!! 编年张 加此QQ492207858 chuckdouble thx221 wrote: 有没有哪位大侠做内皮细胞方面的信号转导的???帮忙给提供几个通路做参考!或者给我点相关
hbsyliuyang rt 请不吝赐教 感谢! david_xmu 没什么太大的区别,另外内皮细胞在不同的培养基中培养可能会有形态上的区别 hbsyliuyang 谢谢这位达人! 拜 本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴 师兄微信号:shixiongcoming
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