- ￥8319
- Lifeline Cell Technology
- 美国
- FC-0001
- 2026年01月18日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Human Dermal Fibroblasts – Neonatal, Primary
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
相关产品： 培养试剂 成纤维细胞培养基
Lifeline® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Lifeline® Dermal Fibroblasts – Neonatal are isolated from neonatal human foreskin and cryopreserved as primary cells to ensure the highest viability and plating efficiency.
- Our skin fibroblasts are quality tested in FibroLife® S2 or FibroLife Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
- Lifeline® dermal fibroblasts tested in both FibroLife® Serum-Free Medium and FibroLife® S2 Medium display optimal growth at rates equal to or greater than classical serum-supplemented medium.
500,000 cells per vial
Lifeline® dermal fibroblasts – neonatal are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
Dermal fibroblasts are quality tested for:
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
- Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
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文献和实验Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups
Neonatal rat ventricular myocytes (NRVM) and fibroblasts (FBs) serve as in vitro models for studying fundamental mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Both cell types are relatively
Isolation and Culture of Skin Fibroblasts
of fibrotic skin diseases. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of adult human fibroblasts. We also provide protocols for constructing three-dimensional dermal and skin equivalent culture models.
. We have recently shown that multipotent dermal stem cells isolated from human neonatal foreskins are able to differentiate to multiple cell lineages, including pigmented melanocytes. The dermal stem cells grow as three-dimensional spheres in human embryonic stem
