- ￥4530
- Lifeline Cell Technology
- 美国
- FC-0024
- 2025年12月30日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Normal Human Dermal Fibroblasts — Adult, Primary
- 细胞类型：
人正常原代细胞
- 物种来源：
人源
- 器官来源：
人源
- 运输方式：
干冰或液氮
- 年限：
液氮储存10年以上
- 生长状态：
冻存
- 规格：
500,000 cells/vial
相关产品： 培养试剂 成纤维细胞培养基
Lifeline® normal Human Dermal Fibroblasts – Adult (HDFa) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Lifeline® Dermal Fibroblasts are cryopreserved as primary cells to ensure the highest viability and plating efficiency.
Our fibroblasts are quality tested in both FibroLife® Serum-Free Medium and FibroLife® S2 Medium to ensure:
- Optimal growth at rates equal to or greater than classical serum-supplemented medium
- Proper growth and morphology over a period of at least 15 population doublings.
500,000 cells per vial
Adult Dermal Fibroblasts are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline® FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.
Dermal fibroblasts are quality tested for:
- Cell Viability: Minimum 70% viability when thawed from cryopreservation
- Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
- Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR
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- 作者
- 内容
- 询问日期
文献和实验Isolation and Culture of Skin Fibroblasts
of fibrotic skin diseases. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of adult human fibroblasts. We also provide protocols for constructing three-dimensional dermal and skin equivalent culture models.
Novel Methods for the Investigation of Human Hypertrophic Scarring and Other Dermal Fibrosis
by progressive deposition of collagen that occurs with high frequency in adult dermal wounds following traumatic or thermal injury. Increased levels of transforming growth factor-β1 (TGF-β1 ), decreased expression of small leucine-rich proteoglycans (SLRPs
Adult Cell Fate Reprogramming: Converting Liver to Pancreas
of some differentiated cells to be converted into a different cell type via a process termed developmental redirection or adult cells reprogramming. For example, abundant human cell types, such as dermal fibroblasts and adipocytes, could potentially be harvested
