- ￥4475
- Leading Biology
- 0.156-10 ng/mL
- EK00787E
- 美国
- 2025年11月16日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
安诺伦（北京）生物科技有限公司
- 检测范围：
0.156-10 ng/mL
- 检测方法：
Spectrophotometrical
- 应用：
Competitive
- 适应物种：
General
- 样本：
This immunoassay kit allows for the in vitro quantitative determination in Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
- 规格：
96T
试剂盒组分：Assay Plate, Standard, Sample Diluent, Assay Diluent A&B, Detection Reagent A&B, Wash Buffer, Substrate, Stop Solution.
功能：Sphingosine (2-amino-4-octadecene-1,3-diol) is an 18-carbon amino alcohol with an unsaturated hydrocarbon chain, which forms a primary part of sphingolipids, a class of cell membrane lipids that include sphingomyelin, an important phospholipid.
储存条件：Store at +4°C. Please refer to protocols.
产品概述：General SPH/Sphingosine ELISA Kit is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit designed for the quantitative measurement of SPH in biological samples. Our rigorously validated, ready-to-go General SPH/Sphingosine ELISA Kit use a simple protocol that easily integrates into your current processes, allowing you to rapidly and accurately detect SPH in your samples. Appropriate sample types may include Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine.
Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested several times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in several separate assays to assess inter-assay precision. Assays were performed by at least two technicians using two lots of components.
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文献和实验A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
the cloning efficiency of DNA fragments. Calculate the optimal reaction conditions to obtain 5 µg of DNA of the fragment of interest: Amount of DNA = 5 µg x (size of fragment)/ (total plasmid size). Use 10 units
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