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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性:
单克隆
- 适应物种:
Human, Pig
- 库存:
100
- 宿主:
Mouse
- 应用范围:
IHC-P, IP, Flo
- 靶点:
Recognizes the CD62P cell surface antigen, a 140kD glycoprotein also known as P-selectin. CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. To ...
- 规格:
50 μg
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文献和实验The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
2-4 selectin分子的结构模式图 表2-3 selectin 家族的组成、分布及其相应配体 selectin家族成员 分布 分子量(kDa) 配体 L-selectin(CD62L,LECAM-1) 白细胞 75~80 PNAd LAM Mel14(小鼠) S-Lewis x P-selectin 血管内皮细胞,血小板 140 S-Lewis x (CD62P,GMP-140,PADGEM) (凝血酶、组胺、白三烯刺激后从α颗粒内与质膜融合而表达
Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets
two times in PBS and resuspend cell pellet in PBS. Cells may be stored at 4� for up to 8 hours. 7. Stain activated platelets by direct immunofluorescence using CD62P-FITC, catalog number 555523 (31794X), at 20 �/test, and compare with resting platelets
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