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PLA2G16 / HRASLS3 Antibody (N-

Terminus)
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  • ¥495
  • Leading Biology
  • APG01055G
  • 2025年10月23日
  • IHC-P, E
  • Goat
  • Human, Rabbit, Monkey
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

    • 适应物种

      Human, Rabbit, Monkey

    • 库存

      100

    • 宿主

      Goat

    • 应用范围

      IHC-P, E

    • 靶点

      Human PLA2G16. Reported variants represent identical protein:NP_009000.2 and NP_001121675.1).

    • 规格

      50 μg

    Prouduct: We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PLA2G16 / HRASLS3 Antibody (N-Terminus). Functiong: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue (By similarity). N- and O- acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity. Summary: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphatidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Specifically catalyzes the release of fatty acids from phospholipids in adipose tissue (By similarity). N- and O- acylation activity is hardly detectable. Might decrease protein phosphatase 2A (PP2A) activity.

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    图标文献和实验
    相关实验
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      标本。 2. 加20ul 1 N HC I ,盖紧,上下混匀。 2 - 8 ℃ 放置60± 2 分钟。 3. 加20ul 1 N NaOH, 盖紧,上下混匀。 4. 即用,或放-20/-70 ℃ 保存3 天。计算结果时乘 以稀释倍数 50 。 ( 注意:不同的标本E2F1 的水平可能有较大差异,请根据实际情况灵活掌握稀释度) 5. 细胞培养上清或组织匀浆 10 倍稀释 (410ul 的标本

    • Monoclonal Antibody Production Protocol

      the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series

    • monoclonal antibody production fusion

      , 50 ml + NaOH, 1N, 100 µl-> heat to 37ѓC, add ddH2 O up to 250 ml   上一篇:Antibody Conjugation Protocol   下一篇:Expanding Hybridoma Clones

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