- ￥495
- Leading Biology
- APR02384G
- 2025年10月21日
- WB, IHC-P, ICC
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 适应物种：
Human, Mouse
- 库存：
100
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, ICC
- 靶点：
16 amino acid peptide from near the center of human TIM-4
- 规格：
50 μg
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文献和实验Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane 3) Block overnight @4C in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 (important if you are re-using
RUVKUN Antibody Staining Protocol revised 10/29/90
amts!, don't use up sample) with 1% toluidine blue. Rinse several times in buffer B. Compare two samples at 50X under dissecting scope. If animals are " open" , + RNAse animals will have very little staining of internal tissues. ANTIBODY INCUBATIONS
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