Vimentin (Mesenchymal Cell Marker) Antibody - Without BSA and Azide产品图

Vimentin (Mesenchymal Cell Ma

rker) Antibody - Without BSA and Azide
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  • $495
  • Leading Biology
  • AMM00425G
  • 2025年10月11日
  • WB, IHC-P, IF, FC
  • Mouse
  • Human, Pig, Chicken, Goat, Cow, Dog, Cat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 亚型

      Mouse / IgG1

    • 保存条件

      Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

    • 克隆性

      单克隆

    • 适应物种

      Human, Pig, Chicken, Goat, Cow, Dog, Cat

    • 库存

      100

    • 宿主

      Mouse

    • 应用范围

      WB, IHC-P, IF, FC

    • 规格

      100 μg

    Prouduct: We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Vimentin (Mesenchymal Cell Marker) Antibody - Without BSA and Azide. Functiong: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Summary: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP’s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.ĀAnti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

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    图标文献和实验
    相关实验
    • Monoclonal Antibody Production Protocol

      - PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase

    • Cell Fusion and Selection

      - Blocking solution = 2 BSA (type V ) in PBS. (Add 0.02 azide for longer storage.) - Elisa buffer = 2 BSA + 0.1 Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish

    • Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry

      antibody against surface marker of interest 0.1% paraformaldehyde in PBS 70% EtOH in glycerol (stored at 4°C) staining buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 m g/ml solution with 100 U/ml DNase-free

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