- $395
- Leading Biology
- AMM00945G
- 2025年09月22日
- IHC, IF, FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Mouse / IgG1, kappa
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 克隆性：
单克隆
- 适应物种：
Human
- 库存：
100
- 宿主：
Mouse
- 应用范围：
IHC, IF, FC
- 规格：
50 ug
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文献和实验Antibody Staining of Imaginal Discs
minutes each wash. 7. Replace last wash with 452 ul Tri-PBS, 5 ul 10% BSA, and 40 ul normal goat serum (NGS); rock for 30 minutes. 8. Add primary antibody directly to the tube and rock overnight at room temperature. 9. Wash tissues 5 times
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
- Blocking solution = 2 BSA (type V ) in PBS. (Add 0.02 azide for longer storage.) - Elisa buffer = 2 BSA + 0.1 Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish
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